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Reevaluating the Role of Acanthamoeba Proteases in Tissue Invasion: Observation of Cytopathogenic Mechanisms on MDCK Cell Monolayers and Hamster Corneal Cells
المؤلفون المشاركون
Martínez-Palomo, Adolfo
Talamás-Rohana, Patricia
Hernández-Ramírez, V. I.
Omaña-Molina, Maritza
Lorenzo-Morales, Jacob
Cristóbal-Ramos, Ana Ruth
Méndez Cruz, Adolfo René
González-Robles, Arturo
Salazar-Villatoro, Lizbeth
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-01-01
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
The morphological analysis of the cytopathic effect on MDCK cell monolayers and hamster cornea and qualitative and quantitative analyses of conditioned medium and proteases were evaluated and compared between two strains of Acanthamoeba genotype T4.
Further than highlighting the biological differences found between both strains, the most important observation in this study was the fact that proteases both in total extracts and in conditioned medium are apparently not determinant in tissue destruction.
An interestingly finding was that no lysis of corneal tissue was observed as it was previously suggested.
These results, together with previous studies, allow us to conclude that the invasion and disruption of corneal tissue is performed by the penetration of the amoebae through cell junctions, either by the action of proteases promoting cellular separation but not by their destruction and/or a mechanical effect exerted by amoebae.
Therefore, contact-dependent mechanisms in Acanthamoeba pathogenesis are more relevant than it has been previously considered.
This is supported because the phagocytosis of recently detached cells as well as those attached to the corneal epithelium leads to the modification of the cellular architecture facilitating the migration and destruction of deeper layers of the corneal epithelium.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Omaña-Molina, Maritza& González-Robles, Arturo& Salazar-Villatoro, Lizbeth& Lorenzo-Morales, Jacob& Cristóbal-Ramos, Ana Ruth& Hernández-Ramírez, V. I.…[et al.]. 2013. Reevaluating the Role of Acanthamoeba Proteases in Tissue Invasion: Observation of Cytopathogenic Mechanisms on MDCK Cell Monolayers and Hamster Corneal Cells. BioMed Research International،Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-1030563
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Omaña-Molina, Maritza…[et al.]. Reevaluating the Role of Acanthamoeba Proteases in Tissue Invasion: Observation of Cytopathogenic Mechanisms on MDCK Cell Monolayers and Hamster Corneal Cells. BioMed Research International No. 2013 (2013), pp.1-13.
https://search.emarefa.net/detail/BIM-1030563
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Omaña-Molina, Maritza& González-Robles, Arturo& Salazar-Villatoro, Lizbeth& Lorenzo-Morales, Jacob& Cristóbal-Ramos, Ana Ruth& Hernández-Ramírez, V. I.…[et al.]. Reevaluating the Role of Acanthamoeba Proteases in Tissue Invasion: Observation of Cytopathogenic Mechanisms on MDCK Cell Monolayers and Hamster Corneal Cells. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-1030563
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1030563
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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