Reevaluating the Role of Acanthamoeba Proteases in Tissue Invasion: Observation of Cytopathogenic Mechanisms on MDCK Cell Monolayers and Hamster Corneal Cells
Joint Authors
Martínez-Palomo, Adolfo
Talamás-Rohana, Patricia
Hernández-Ramírez, V. I.
Omaña-Molina, Maritza
Lorenzo-Morales, Jacob
Cristóbal-Ramos, Ana Ruth
Méndez Cruz, Adolfo René
González-Robles, Arturo
Salazar-Villatoro, Lizbeth
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-01-01
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
The morphological analysis of the cytopathic effect on MDCK cell monolayers and hamster cornea and qualitative and quantitative analyses of conditioned medium and proteases were evaluated and compared between two strains of Acanthamoeba genotype T4.
Further than highlighting the biological differences found between both strains, the most important observation in this study was the fact that proteases both in total extracts and in conditioned medium are apparently not determinant in tissue destruction.
An interestingly finding was that no lysis of corneal tissue was observed as it was previously suggested.
These results, together with previous studies, allow us to conclude that the invasion and disruption of corneal tissue is performed by the penetration of the amoebae through cell junctions, either by the action of proteases promoting cellular separation but not by their destruction and/or a mechanical effect exerted by amoebae.
Therefore, contact-dependent mechanisms in Acanthamoeba pathogenesis are more relevant than it has been previously considered.
This is supported because the phagocytosis of recently detached cells as well as those attached to the corneal epithelium leads to the modification of the cellular architecture facilitating the migration and destruction of deeper layers of the corneal epithelium.
American Psychological Association (APA)
Omaña-Molina, Maritza& González-Robles, Arturo& Salazar-Villatoro, Lizbeth& Lorenzo-Morales, Jacob& Cristóbal-Ramos, Ana Ruth& Hernández-Ramírez, V. I.…[et al.]. 2013. Reevaluating the Role of Acanthamoeba Proteases in Tissue Invasion: Observation of Cytopathogenic Mechanisms on MDCK Cell Monolayers and Hamster Corneal Cells. BioMed Research International،Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-1030563
Modern Language Association (MLA)
Omaña-Molina, Maritza…[et al.]. Reevaluating the Role of Acanthamoeba Proteases in Tissue Invasion: Observation of Cytopathogenic Mechanisms on MDCK Cell Monolayers and Hamster Corneal Cells. BioMed Research International No. 2013 (2013), pp.1-13.
https://search.emarefa.net/detail/BIM-1030563
American Medical Association (AMA)
Omaña-Molina, Maritza& González-Robles, Arturo& Salazar-Villatoro, Lizbeth& Lorenzo-Morales, Jacob& Cristóbal-Ramos, Ana Ruth& Hernández-Ramírez, V. I.…[et al.]. Reevaluating the Role of Acanthamoeba Proteases in Tissue Invasion: Observation of Cytopathogenic Mechanisms on MDCK Cell Monolayers and Hamster Corneal Cells. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-1030563
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030563