Enhanced Production of Acarbose and Concurrently Reduced Formation of Impurity C by Addition of Validamine in Fermentation of Actinoplanes utahensis ZJB-08196
المؤلفون المشاركون
Xue, Ya-Ping
Qin, Jun-Wei
Wang, Yuan-Shan
Zheng, Yu-Guo
Wang, Yajun
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-01-22
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Commercial production of acarbose is exclusively via done microbial fermentation with strains from the genera of Actinoplanes.
The addition of C7N-aminocyclitols for enhanced production of acarbose and concurrently reduced formation of impurity C by cultivation of A.
utahensis ZJB-08196 in 500-mL shake flasks was investigated, and validamine was found to be the most effective strategy.
Under the optimal conditions of validamine addition, acarbose titer was increased from 3560±128 mg/L to 4950±156 mg/L, and impurity C concentration was concurrently decreased from 289±24 mg/L to 107±29 mg/L in batch fermentation after 168 h of cultivation.
A further fed-batch experiment coupled with the addition of validamine (20 mg/L) in the fermentation medium prior to inoculation was designed to enhance the production of acarbose.
When twice feedings of a mixture of 6 g/L glucose, 14 g/L maltose, and 9 g/L soybean flour were performed at 72 h and 96 h, acarbose titer reached 6606±103 mg/L and impurity C concentration was only 212±12 mg/L at 168 h of cultivation.
Acarbose titer and proportion of acarbose/impurity C increased by 85.6% and 152.9% when compared with control experiments.
This work demonstrates for the first time that validamine addition is a simple and effective strategy for increasing acarbose production and reducing impurity C formation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Xue, Ya-Ping& Qin, Jun-Wei& Wang, Yajun& Wang, Yuan-Shan& Zheng, Yu-Guo. 2013. Enhanced Production of Acarbose and Concurrently Reduced Formation of Impurity C by Addition of Validamine in Fermentation of Actinoplanes utahensis ZJB-08196. BioMed Research International،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1030819
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Xue, Ya-Ping…[et al.]. Enhanced Production of Acarbose and Concurrently Reduced Formation of Impurity C by Addition of Validamine in Fermentation of Actinoplanes utahensis ZJB-08196. BioMed Research International No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1030819
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Xue, Ya-Ping& Qin, Jun-Wei& Wang, Yajun& Wang, Yuan-Shan& Zheng, Yu-Guo. Enhanced Production of Acarbose and Concurrently Reduced Formation of Impurity C by Addition of Validamine in Fermentation of Actinoplanes utahensis ZJB-08196. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1030819
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1030819
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر