Enhanced Production of Acarbose and Concurrently Reduced Formation of Impurity C by Addition of Validamine in Fermentation of Actinoplanes utahensis ZJB-08196
Joint Authors
Xue, Ya-Ping
Qin, Jun-Wei
Wang, Yuan-Shan
Zheng, Yu-Guo
Wang, Yajun
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-01-22
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Commercial production of acarbose is exclusively via done microbial fermentation with strains from the genera of Actinoplanes.
The addition of C7N-aminocyclitols for enhanced production of acarbose and concurrently reduced formation of impurity C by cultivation of A.
utahensis ZJB-08196 in 500-mL shake flasks was investigated, and validamine was found to be the most effective strategy.
Under the optimal conditions of validamine addition, acarbose titer was increased from 3560±128 mg/L to 4950±156 mg/L, and impurity C concentration was concurrently decreased from 289±24 mg/L to 107±29 mg/L in batch fermentation after 168 h of cultivation.
A further fed-batch experiment coupled with the addition of validamine (20 mg/L) in the fermentation medium prior to inoculation was designed to enhance the production of acarbose.
When twice feedings of a mixture of 6 g/L glucose, 14 g/L maltose, and 9 g/L soybean flour were performed at 72 h and 96 h, acarbose titer reached 6606±103 mg/L and impurity C concentration was only 212±12 mg/L at 168 h of cultivation.
Acarbose titer and proportion of acarbose/impurity C increased by 85.6% and 152.9% when compared with control experiments.
This work demonstrates for the first time that validamine addition is a simple and effective strategy for increasing acarbose production and reducing impurity C formation.
American Psychological Association (APA)
Xue, Ya-Ping& Qin, Jun-Wei& Wang, Yajun& Wang, Yuan-Shan& Zheng, Yu-Guo. 2013. Enhanced Production of Acarbose and Concurrently Reduced Formation of Impurity C by Addition of Validamine in Fermentation of Actinoplanes utahensis ZJB-08196. BioMed Research International،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1030819
Modern Language Association (MLA)
Xue, Ya-Ping…[et al.]. Enhanced Production of Acarbose and Concurrently Reduced Formation of Impurity C by Addition of Validamine in Fermentation of Actinoplanes utahensis ZJB-08196. BioMed Research International No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1030819
American Medical Association (AMA)
Xue, Ya-Ping& Qin, Jun-Wei& Wang, Yajun& Wang, Yuan-Shan& Zheng, Yu-Guo. Enhanced Production of Acarbose and Concurrently Reduced Formation of Impurity C by Addition of Validamine in Fermentation of Actinoplanes utahensis ZJB-08196. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1030819
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030819