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In Vitro Large Scale Production of Human Mature Red Blood Cells from Hematopoietic Stem Cells by Coculturing with Human Fetal Liver Stromal Cells
المؤلفون المشاركون
Li, Yanhua
Xi, Jiafei
Wang, Ruoyong
Nan, Xue
He, Lijuan
Chen, Lin
Yue, Wen
Pei, Xuetao
Zhang, Peng
Wang, Yunfang
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-01-30
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
In vitro models of human erythropoiesis are useful in studying the mechanisms of erythroid differentiation in normal and pathological conditions.
Here we describe an erythroid liquid culture system starting from cord blood derived hematopoietic stem cells (HSCs).
HSCs were cultured for more than 50 days in erythroid differentiation conditions and resulted in a more than 109-fold expansion within 50 days under optimal conditions.
Homogeneous erythroid cells were characterized by cell morphology, flow cytometry, and hematopoietic colony assays.
Furthermore, terminal erythroid maturation was improved by cosculturing with human fetal liver stromal cells.
Cocultured erythroid cells underwent multiple maturation events, including decrease in size, increase in glycophorin A expression, and nuclear condensation.
This process resulted in extrusion of the pycnotic nuclei in up to 80% of the cells.
Importantly, they possessed the capacity to express the adult definitive β-globin chain upon further maturation.
We also show that the oxygen equilibrium curves of the cord blood-differentiated red blood cells (RBCs) are comparable to normal RBCs.
The large number and purity of erythroid cells and RBCs produced from cord blood make this method useful for fundamental research in erythroid development, and they also provide a basis for future production of available RBCs for transfusion.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Xi, Jiafei& Li, Yanhua& Wang, Ruoyong& Wang, Yunfang& Nan, Xue& He, Lijuan…[et al.]. 2013. In Vitro Large Scale Production of Human Mature Red Blood Cells from Hematopoietic Stem Cells by Coculturing with Human Fetal Liver Stromal Cells. BioMed Research International،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-1030941
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Xi, Jiafei…[et al.]. In Vitro Large Scale Production of Human Mature Red Blood Cells from Hematopoietic Stem Cells by Coculturing with Human Fetal Liver Stromal Cells. BioMed Research International No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-1030941
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Xi, Jiafei& Li, Yanhua& Wang, Ruoyong& Wang, Yunfang& Nan, Xue& He, Lijuan…[et al.]. In Vitro Large Scale Production of Human Mature Red Blood Cells from Hematopoietic Stem Cells by Coculturing with Human Fetal Liver Stromal Cells. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-1030941
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1030941
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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