In Vitro Large Scale Production of Human Mature Red Blood Cells from Hematopoietic Stem Cells by Coculturing with Human Fetal Liver Stromal Cells
Joint Authors
Li, Yanhua
Xi, Jiafei
Wang, Ruoyong
Nan, Xue
He, Lijuan
Chen, Lin
Yue, Wen
Pei, Xuetao
Zhang, Peng
Wang, Yunfang
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-01-30
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
In vitro models of human erythropoiesis are useful in studying the mechanisms of erythroid differentiation in normal and pathological conditions.
Here we describe an erythroid liquid culture system starting from cord blood derived hematopoietic stem cells (HSCs).
HSCs were cultured for more than 50 days in erythroid differentiation conditions and resulted in a more than 109-fold expansion within 50 days under optimal conditions.
Homogeneous erythroid cells were characterized by cell morphology, flow cytometry, and hematopoietic colony assays.
Furthermore, terminal erythroid maturation was improved by cosculturing with human fetal liver stromal cells.
Cocultured erythroid cells underwent multiple maturation events, including decrease in size, increase in glycophorin A expression, and nuclear condensation.
This process resulted in extrusion of the pycnotic nuclei in up to 80% of the cells.
Importantly, they possessed the capacity to express the adult definitive β-globin chain upon further maturation.
We also show that the oxygen equilibrium curves of the cord blood-differentiated red blood cells (RBCs) are comparable to normal RBCs.
The large number and purity of erythroid cells and RBCs produced from cord blood make this method useful for fundamental research in erythroid development, and they also provide a basis for future production of available RBCs for transfusion.
American Psychological Association (APA)
Xi, Jiafei& Li, Yanhua& Wang, Ruoyong& Wang, Yunfang& Nan, Xue& He, Lijuan…[et al.]. 2013. In Vitro Large Scale Production of Human Mature Red Blood Cells from Hematopoietic Stem Cells by Coculturing with Human Fetal Liver Stromal Cells. BioMed Research International،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-1030941
Modern Language Association (MLA)
Xi, Jiafei…[et al.]. In Vitro Large Scale Production of Human Mature Red Blood Cells from Hematopoietic Stem Cells by Coculturing with Human Fetal Liver Stromal Cells. BioMed Research International No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-1030941
American Medical Association (AMA)
Xi, Jiafei& Li, Yanhua& Wang, Ruoyong& Wang, Yunfang& Nan, Xue& He, Lijuan…[et al.]. In Vitro Large Scale Production of Human Mature Red Blood Cells from Hematopoietic Stem Cells by Coculturing with Human Fetal Liver Stromal Cells. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-1030941
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030941