Development of a Novel System for Mass Spectrometric Analysis of Cancer-Associated Fucosylation in Plasma α1-Acid Glycoprotein
المؤلفون المشاركون
Kuwano, Hiroyuki
Asao, Takayuki
Yazawa, Shin
Nishimura, Toyo
Hayashi, Takashi
Shimaoka, Hideyuki
Saniabadi, Abby R.
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-02-13
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Human plasma α1-acid glycoprotein (AGP) from cancer patients and healthy volunteers was purified by sequential application of ion-exchange columns, and N-linked glycans enzymatically released from AGP were labeled and applied to a mass spectrometer.
Additionally, a novel software system for use in combination with a mass spectrometer to determine N-linked glycans in AGP was developed.
A database with 607 glycans including 453 different glycan structures that were theoretically predicted to be present in AGP was prepared for designing the software called AGPAS.
This AGPAS was applied to determine relative abundance of each glycan in the AGP molecules based on mass spectra.
It was found that the relative abundance of fucosylated glycans in tri- and tetra-antennary structures (FUCAGP) was significantly higher in cancer patients as compared with the healthy group (P<0.001).
Furthermore, extremely elevated levels of FUCAGP were found specifically in patients with a poor prognosis but not in patients with a good prognosis.
In conclusion, the present software system allowed rapid determination of the primary structures of AGP glycans.
The fucosylated glycans as novel tumor markers have clinical relevance in the diagnosis and assessment of cancer progression as well as patient prognosis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Asao, Takayuki& Yazawa, Shin& Nishimura, Toyo& Hayashi, Takashi& Shimaoka, Hideyuki & Saniabadi, Abby R.…[et al.]. 2013. Development of a Novel System for Mass Spectrometric Analysis of Cancer-Associated Fucosylation in Plasma α1-Acid Glycoprotein. BioMed Research International،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1030973
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Asao, Takayuki…[et al.]. Development of a Novel System for Mass Spectrometric Analysis of Cancer-Associated Fucosylation in Plasma α1-Acid Glycoprotein. BioMed Research International No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1030973
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Asao, Takayuki& Yazawa, Shin& Nishimura, Toyo& Hayashi, Takashi& Shimaoka, Hideyuki & Saniabadi, Abby R.…[et al.]. Development of a Novel System for Mass Spectrometric Analysis of Cancer-Associated Fucosylation in Plasma α1-Acid Glycoprotein. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1030973
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1030973
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر