Development of a Novel System for Mass Spectrometric Analysis of Cancer-Associated Fucosylation in Plasma α1-Acid Glycoprotein
Joint Authors
Kuwano, Hiroyuki
Asao, Takayuki
Yazawa, Shin
Nishimura, Toyo
Hayashi, Takashi
Shimaoka, Hideyuki
Saniabadi, Abby R.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-02-13
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Human plasma α1-acid glycoprotein (AGP) from cancer patients and healthy volunteers was purified by sequential application of ion-exchange columns, and N-linked glycans enzymatically released from AGP were labeled and applied to a mass spectrometer.
Additionally, a novel software system for use in combination with a mass spectrometer to determine N-linked glycans in AGP was developed.
A database with 607 glycans including 453 different glycan structures that were theoretically predicted to be present in AGP was prepared for designing the software called AGPAS.
This AGPAS was applied to determine relative abundance of each glycan in the AGP molecules based on mass spectra.
It was found that the relative abundance of fucosylated glycans in tri- and tetra-antennary structures (FUCAGP) was significantly higher in cancer patients as compared with the healthy group (P<0.001).
Furthermore, extremely elevated levels of FUCAGP were found specifically in patients with a poor prognosis but not in patients with a good prognosis.
In conclusion, the present software system allowed rapid determination of the primary structures of AGP glycans.
The fucosylated glycans as novel tumor markers have clinical relevance in the diagnosis and assessment of cancer progression as well as patient prognosis.
American Psychological Association (APA)
Asao, Takayuki& Yazawa, Shin& Nishimura, Toyo& Hayashi, Takashi& Shimaoka, Hideyuki & Saniabadi, Abby R.…[et al.]. 2013. Development of a Novel System for Mass Spectrometric Analysis of Cancer-Associated Fucosylation in Plasma α1-Acid Glycoprotein. BioMed Research International،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1030973
Modern Language Association (MLA)
Asao, Takayuki…[et al.]. Development of a Novel System for Mass Spectrometric Analysis of Cancer-Associated Fucosylation in Plasma α1-Acid Glycoprotein. BioMed Research International No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1030973
American Medical Association (AMA)
Asao, Takayuki& Yazawa, Shin& Nishimura, Toyo& Hayashi, Takashi& Shimaoka, Hideyuki & Saniabadi, Abby R.…[et al.]. Development of a Novel System for Mass Spectrometric Analysis of Cancer-Associated Fucosylation in Plasma α1-Acid Glycoprotein. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1030973
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030973