Performance of Glutamate Dehydrogenase and Triose Phosphate Isomerase Genes in the Analysis of Genotypic Variability of Isolates of Giardia duodenalis from Livestocks
المؤلفون المشاركون
Ueira-Vieira, Carlos
Fava, Natália M. N.
Soares, Rodrigo M.
Scalia, Luana A. M.
Kalapothakis, Evanguedes
Pena, Isabella F.
Faria, Elaine S. M.
Cunha, Maria J.
Couto, Talles R.
Cury, Márcia Cristina
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-11-06
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Giardia duodenalis is a small intestinal protozoan parasite of several terrestrial vertebrates.
This work aims to assess the genotypic variability of Giardia duodenalis isolates from cattle, sheep and pigs in the Southeast of Brazil, by comparing the standard characterization between glutamate dehydrogenase (gdh) and triose phosphate isomerase (tpi) primers.
Fecal samples from the three groups of animals were analyzed using the zinc sulphate centrifugal flotation technique.
Out of 59 positive samples, 30 were from cattle, 26 from sheep and 3 from pigs.
Cyst pellets were stored and submitted to PCR and nested-PCR reactions with gdh and tpi primers.
Fragment amplification of gdh and tpi genes was observed in 25 (42.4%) and 36 (61.0%) samples, respectively.
Regarding the sequencing, 24 sequences were obtained with gdh and 20 with tpi.
For both genes, there was a prevalence of E specific species assemblage, although some isolates have been identified as A and B, by the tpi sequencing.
This has also shown a larger number of heterogeneous sequences, which have been attribute to mixed infections between assemblages B and E.
The largest variability of inter-assemblage associated to the frequency of heterogeneity provided by tpi sequencing reinforces the polymorphic nature of this gene and makes it an excellent target for studies on molecular epidemiology.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Fava, Natália M. N.& Soares, Rodrigo M.& Scalia, Luana A. M.& Kalapothakis, Evanguedes& Pena, Isabella F.& Ueira-Vieira, Carlos…[et al.]. 2013. Performance of Glutamate Dehydrogenase and Triose Phosphate Isomerase Genes in the Analysis of Genotypic Variability of Isolates of Giardia duodenalis from Livestocks. BioMed Research International،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1031048
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Fava, Natália M. N.…[et al.]. Performance of Glutamate Dehydrogenase and Triose Phosphate Isomerase Genes in the Analysis of Genotypic Variability of Isolates of Giardia duodenalis from Livestocks. BioMed Research International No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1031048
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Fava, Natália M. N.& Soares, Rodrigo M.& Scalia, Luana A. M.& Kalapothakis, Evanguedes& Pena, Isabella F.& Ueira-Vieira, Carlos…[et al.]. Performance of Glutamate Dehydrogenase and Triose Phosphate Isomerase Genes in the Analysis of Genotypic Variability of Isolates of Giardia duodenalis from Livestocks. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1031048
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1031048
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر