Performance of Glutamate Dehydrogenase and Triose Phosphate Isomerase Genes in the Analysis of Genotypic Variability of Isolates of Giardia duodenalis from Livestocks
Joint Authors
Ueira-Vieira, Carlos
Fava, Natália M. N.
Soares, Rodrigo M.
Scalia, Luana A. M.
Kalapothakis, Evanguedes
Pena, Isabella F.
Faria, Elaine S. M.
Cunha, Maria J.
Couto, Talles R.
Cury, Márcia Cristina
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-06
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Giardia duodenalis is a small intestinal protozoan parasite of several terrestrial vertebrates.
This work aims to assess the genotypic variability of Giardia duodenalis isolates from cattle, sheep and pigs in the Southeast of Brazil, by comparing the standard characterization between glutamate dehydrogenase (gdh) and triose phosphate isomerase (tpi) primers.
Fecal samples from the three groups of animals were analyzed using the zinc sulphate centrifugal flotation technique.
Out of 59 positive samples, 30 were from cattle, 26 from sheep and 3 from pigs.
Cyst pellets were stored and submitted to PCR and nested-PCR reactions with gdh and tpi primers.
Fragment amplification of gdh and tpi genes was observed in 25 (42.4%) and 36 (61.0%) samples, respectively.
Regarding the sequencing, 24 sequences were obtained with gdh and 20 with tpi.
For both genes, there was a prevalence of E specific species assemblage, although some isolates have been identified as A and B, by the tpi sequencing.
This has also shown a larger number of heterogeneous sequences, which have been attribute to mixed infections between assemblages B and E.
The largest variability of inter-assemblage associated to the frequency of heterogeneity provided by tpi sequencing reinforces the polymorphic nature of this gene and makes it an excellent target for studies on molecular epidemiology.
American Psychological Association (APA)
Fava, Natália M. N.& Soares, Rodrigo M.& Scalia, Luana A. M.& Kalapothakis, Evanguedes& Pena, Isabella F.& Ueira-Vieira, Carlos…[et al.]. 2013. Performance of Glutamate Dehydrogenase and Triose Phosphate Isomerase Genes in the Analysis of Genotypic Variability of Isolates of Giardia duodenalis from Livestocks. BioMed Research International،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1031048
Modern Language Association (MLA)
Fava, Natália M. N.…[et al.]. Performance of Glutamate Dehydrogenase and Triose Phosphate Isomerase Genes in the Analysis of Genotypic Variability of Isolates of Giardia duodenalis from Livestocks. BioMed Research International No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1031048
American Medical Association (AMA)
Fava, Natália M. N.& Soares, Rodrigo M.& Scalia, Luana A. M.& Kalapothakis, Evanguedes& Pena, Isabella F.& Ueira-Vieira, Carlos…[et al.]. Performance of Glutamate Dehydrogenase and Triose Phosphate Isomerase Genes in the Analysis of Genotypic Variability of Isolates of Giardia duodenalis from Livestocks. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1031048
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1031048