Rapid Purification and Procoagulant and Platelet Aggregating Activities of Rhombeobin: A Thrombin-LikeGyroxin-Like Enzyme from Lachesis muta rhombeata Snake Venom
المؤلفون المشاركون
Torres-Huaco, Frank Denis
Werneck, Cláudio C.
Vicente, Cristina Pontes
Vassequi-Silva, Talita
Nery-Diez, Ana Cláudia Coelho
Mendes, Camila B.
Antunes, Edson
Damico, Daniela C. S.
Marangoni, Sérgio
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-08-24
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
We report a rapid purification method using one-step chromatography of SVSP Rhombeobin (LMR-47) from Lachesis muta rhombeata venom and its procoagulant activities and effects on platelet aggregation.
The venom was fractionated by a single chromatographic step in RP-HPLC on a C8 Discovery BIO Wide Pore, showing high degree of molecular homogeneity with molecular mass of 47035.49 Da.
Rhombeobin showed amidolytic activity upon BAρNA, with a broad optimum pH (7–10) and was stable in solution up to 60°C.
The amidolytic activity was inhibited by serine proteinase inhibitors and reducing agents, but not chelating agents.
Rhombeobin showed high coagulant activity on mice plasma and bovine fibrinogen.
The deduced amino acid sequence of Rhombeobin showed homology with other SVSPs, especially with LM-TL (L.
m.
muta) and Gyroxin (C.
d.
terrificus).
Rhombeobin acts, in vitro, as a strong procoagulant enzyme on mice citrated plasma, shortening the APTT and PT tests in adose-dependent manner.
The protein showed, “ex vivo”, a strong defibrinogenating effect with 1 µg/animal.
Lower doses activated the intrinsic and extrinsic coagulation pathways and impaired the platelet aggregation induced by ADP.
Thus, this is the first report of a venom component that produces a venom-induced consumptive coagulopathy (VICC).
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Torres-Huaco, Frank Denis& Werneck, Cláudio C.& Vicente, Cristina Pontes& Vassequi-Silva, Talita& Nery-Diez, Ana Cláudia Coelho& Mendes, Camila B.…[et al.]. 2013. Rapid Purification and Procoagulant and Platelet Aggregating Activities of Rhombeobin: A Thrombin-LikeGyroxin-Like Enzyme from Lachesis muta rhombeata Snake Venom. BioMed Research International،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-1031064
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Torres-Huaco, Frank Denis…[et al.]. Rapid Purification and Procoagulant and Platelet Aggregating Activities of Rhombeobin: A Thrombin-LikeGyroxin-Like Enzyme from Lachesis muta rhombeata Snake Venom. BioMed Research International No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-1031064
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Torres-Huaco, Frank Denis& Werneck, Cláudio C.& Vicente, Cristina Pontes& Vassequi-Silva, Talita& Nery-Diez, Ana Cláudia Coelho& Mendes, Camila B.…[et al.]. Rapid Purification and Procoagulant and Platelet Aggregating Activities of Rhombeobin: A Thrombin-LikeGyroxin-Like Enzyme from Lachesis muta rhombeata Snake Venom. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-1031064
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1031064
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر