Rapid Purification and Procoagulant and Platelet Aggregating Activities of Rhombeobin: A Thrombin-LikeGyroxin-Like Enzyme from Lachesis muta rhombeata Snake Venom
Joint Authors
Torres-Huaco, Frank Denis
Werneck, Cláudio C.
Vicente, Cristina Pontes
Vassequi-Silva, Talita
Nery-Diez, Ana Cláudia Coelho
Mendes, Camila B.
Antunes, Edson
Damico, Daniela C. S.
Marangoni, Sérgio
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-08-24
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
We report a rapid purification method using one-step chromatography of SVSP Rhombeobin (LMR-47) from Lachesis muta rhombeata venom and its procoagulant activities and effects on platelet aggregation.
The venom was fractionated by a single chromatographic step in RP-HPLC on a C8 Discovery BIO Wide Pore, showing high degree of molecular homogeneity with molecular mass of 47035.49 Da.
Rhombeobin showed amidolytic activity upon BAρNA, with a broad optimum pH (7–10) and was stable in solution up to 60°C.
The amidolytic activity was inhibited by serine proteinase inhibitors and reducing agents, but not chelating agents.
Rhombeobin showed high coagulant activity on mice plasma and bovine fibrinogen.
The deduced amino acid sequence of Rhombeobin showed homology with other SVSPs, especially with LM-TL (L.
m.
muta) and Gyroxin (C.
d.
terrificus).
Rhombeobin acts, in vitro, as a strong procoagulant enzyme on mice citrated plasma, shortening the APTT and PT tests in adose-dependent manner.
The protein showed, “ex vivo”, a strong defibrinogenating effect with 1 µg/animal.
Lower doses activated the intrinsic and extrinsic coagulation pathways and impaired the platelet aggregation induced by ADP.
Thus, this is the first report of a venom component that produces a venom-induced consumptive coagulopathy (VICC).
American Psychological Association (APA)
Torres-Huaco, Frank Denis& Werneck, Cláudio C.& Vicente, Cristina Pontes& Vassequi-Silva, Talita& Nery-Diez, Ana Cláudia Coelho& Mendes, Camila B.…[et al.]. 2013. Rapid Purification and Procoagulant and Platelet Aggregating Activities of Rhombeobin: A Thrombin-LikeGyroxin-Like Enzyme from Lachesis muta rhombeata Snake Venom. BioMed Research International،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-1031064
Modern Language Association (MLA)
Torres-Huaco, Frank Denis…[et al.]. Rapid Purification and Procoagulant and Platelet Aggregating Activities of Rhombeobin: A Thrombin-LikeGyroxin-Like Enzyme from Lachesis muta rhombeata Snake Venom. BioMed Research International No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-1031064
American Medical Association (AMA)
Torres-Huaco, Frank Denis& Werneck, Cláudio C.& Vicente, Cristina Pontes& Vassequi-Silva, Talita& Nery-Diez, Ana Cláudia Coelho& Mendes, Camila B.…[et al.]. Rapid Purification and Procoagulant and Platelet Aggregating Activities of Rhombeobin: A Thrombin-LikeGyroxin-Like Enzyme from Lachesis muta rhombeata Snake Venom. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-1031064
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1031064