A Simple Assay to Screen Antimicrobial Compounds Potentiating the Activity of Current Antibiotics
المؤلفون المشاركون
Iqbal, Junaid
Kazmi, Shahana Urooj
Siddiqui, Ruqaiyyah
Khan, Naveed Ahmed
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-4، 4ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-06-20
دولة النشر
مصر
عدد الصفحات
4
التخصصات الرئيسية
الملخص EN
Antibiotic resistance continues to pose a significant problem in the management of bacterial infections, despite advances in antimicrobial chemotherapy and supportive care.
Here, we suggest a simple, inexpensive, and easy-to-perform assay to screen antimicrobial compounds from natural products or synthetic chemical libraries for their potential to work in tandem with the available antibiotics against multiple drug-resistant bacteria.
The aqueous extract of Juglans regia tree bark was tested against representative multiple drug-resistant bacteria in the aforementioned assay to determine whether it potentiates the activity of selected antibiotics.
The aqueous extract of J.
regia bark was added to Mueller-Hinton agar, followed by a lawn of multiple drug-resistant bacteria, Salmonella typhi or enteropathogenic E.
coli.
Next, filter paper discs impregnated with different classes of antibiotics were placed on the agar surface.
Bacteria incubated with extract or antibiotics alone were used as controls.
The results showed a significant increase (>30%) in the zone of inhibition around the aztreonam, cefuroxime, and ampicillin discs compared with bacteria incubated with the antibiotics/extract alone.
In conclusion, our assay is able to detect either synergistic or additive action of J.
regia extract against multiple drug-resistant bacteria when tested with a range of antibiotics.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Iqbal, Junaid& Siddiqui, Ruqaiyyah& Kazmi, Shahana Urooj& Khan, Naveed Ahmed. 2013. A Simple Assay to Screen Antimicrobial Compounds Potentiating the Activity of Current Antibiotics. BioMed Research International،Vol. 2013, no. 2013, pp.1-4.
https://search.emarefa.net/detail/BIM-1031105
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Iqbal, Junaid…[et al.]. A Simple Assay to Screen Antimicrobial Compounds Potentiating the Activity of Current Antibiotics. BioMed Research International No. 2013 (2013), pp.1-4.
https://search.emarefa.net/detail/BIM-1031105
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Iqbal, Junaid& Siddiqui, Ruqaiyyah& Kazmi, Shahana Urooj& Khan, Naveed Ahmed. A Simple Assay to Screen Antimicrobial Compounds Potentiating the Activity of Current Antibiotics. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-4.
https://search.emarefa.net/detail/BIM-1031105
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1031105
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر