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A Simple Assay to Screen Antimicrobial Compounds Potentiating the Activity of Current Antibiotics
Joint Authors
Iqbal, Junaid
Kazmi, Shahana Urooj
Siddiqui, Ruqaiyyah
Khan, Naveed Ahmed
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-06-20
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Abstract EN
Antibiotic resistance continues to pose a significant problem in the management of bacterial infections, despite advances in antimicrobial chemotherapy and supportive care.
Here, we suggest a simple, inexpensive, and easy-to-perform assay to screen antimicrobial compounds from natural products or synthetic chemical libraries for their potential to work in tandem with the available antibiotics against multiple drug-resistant bacteria.
The aqueous extract of Juglans regia tree bark was tested against representative multiple drug-resistant bacteria in the aforementioned assay to determine whether it potentiates the activity of selected antibiotics.
The aqueous extract of J.
regia bark was added to Mueller-Hinton agar, followed by a lawn of multiple drug-resistant bacteria, Salmonella typhi or enteropathogenic E.
coli.
Next, filter paper discs impregnated with different classes of antibiotics were placed on the agar surface.
Bacteria incubated with extract or antibiotics alone were used as controls.
The results showed a significant increase (>30%) in the zone of inhibition around the aztreonam, cefuroxime, and ampicillin discs compared with bacteria incubated with the antibiotics/extract alone.
In conclusion, our assay is able to detect either synergistic or additive action of J.
regia extract against multiple drug-resistant bacteria when tested with a range of antibiotics.
American Psychological Association (APA)
Iqbal, Junaid& Siddiqui, Ruqaiyyah& Kazmi, Shahana Urooj& Khan, Naveed Ahmed. 2013. A Simple Assay to Screen Antimicrobial Compounds Potentiating the Activity of Current Antibiotics. BioMed Research International،Vol. 2013, no. 2013, pp.1-4.
https://search.emarefa.net/detail/BIM-1031105
Modern Language Association (MLA)
Iqbal, Junaid…[et al.]. A Simple Assay to Screen Antimicrobial Compounds Potentiating the Activity of Current Antibiotics. BioMed Research International No. 2013 (2013), pp.1-4.
https://search.emarefa.net/detail/BIM-1031105
American Medical Association (AMA)
Iqbal, Junaid& Siddiqui, Ruqaiyyah& Kazmi, Shahana Urooj& Khan, Naveed Ahmed. A Simple Assay to Screen Antimicrobial Compounds Potentiating the Activity of Current Antibiotics. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-4.
https://search.emarefa.net/detail/BIM-1031105
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1031105