Antibacterial Activity of Defensin PaDef from Avocado Fruit (Persea americana var. drymifolia) Expressed in Endothelial Cells against Escherichia coli and Staphylococcus aureus
المؤلفون المشاركون
López-Meza, Joel Edmundo
Ochoa-Zarzosa, Alejandra
Guzmán-Rodríguez, Jaquelina Julia
López-Gómez, Rodolfo
Suárez-Rodríguez, Luis M.
Rodríguez-Zapata, Luis C.
Salgado-Garciglia, Rafael
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-11-12
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Antimicrobial therapy is a useful tool to control infectious diseases in general and rising antibiotic resistant microorganisms in particular.
Alternative strategies are desirable, and antimicrobial peptides (AMP) represent attractive control agents.
Mexican avocado (Persea americana var.
drymifolia) is used in traditional medicine; however, the AMP production has not been reported in this plant.
We obtained a cDNA library from avocado fruit and clone PaDef was identified, which has a cDNA (249 bp) encoding a protein (78 aa) homologous with plant defensins (>80%).
We expressed the defensin PaDef cDNA (pBME3) in the bovine endothelial cell line BVE-E6E7.
Polyclonal and clonal populations were obtained and their activity was evaluated against Escherichia coli, Staphylococcus aureus, and Candida albicans.
E.
coli viability was inhibited with 100 μg/mL of total protein from clones (>55%).
Also, S.
aureus viability was inhibited from 50 μg/mL total protein (27–38%) but was more evident at 100 μg/mL (52–65%).
This inhibition was higher than the effect showed by polyclonal population (~23%).
Finally, we did not detect activity against C.
albicans.
These results are the first report that shows antimicrobial activity of a defensin produced by avocado and suggest that this AMP could be used in the control of pathogens.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Guzmán-Rodríguez, Jaquelina Julia& López-Gómez, Rodolfo& Suárez-Rodríguez, Luis M.& Salgado-Garciglia, Rafael& Rodríguez-Zapata, Luis C.& Ochoa-Zarzosa, Alejandra…[et al.]. 2013. Antibacterial Activity of Defensin PaDef from Avocado Fruit (Persea americana var. drymifolia) Expressed in Endothelial Cells against Escherichia coli and Staphylococcus aureus. BioMed Research International،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1031173
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Guzmán-Rodríguez, Jaquelina Julia…[et al.]. Antibacterial Activity of Defensin PaDef from Avocado Fruit (Persea americana var. drymifolia) Expressed in Endothelial Cells against Escherichia coli and Staphylococcus aureus. BioMed Research International No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1031173
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Guzmán-Rodríguez, Jaquelina Julia& López-Gómez, Rodolfo& Suárez-Rodríguez, Luis M.& Salgado-Garciglia, Rafael& Rodríguez-Zapata, Luis C.& Ochoa-Zarzosa, Alejandra…[et al.]. Antibacterial Activity of Defensin PaDef from Avocado Fruit (Persea americana var. drymifolia) Expressed in Endothelial Cells against Escherichia coli and Staphylococcus aureus. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1031173
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1031173
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر