![](/images/graphics-bg.png)
Antibacterial Activity of Defensin PaDef from Avocado Fruit (Persea americana var. drymifolia) Expressed in Endothelial Cells against Escherichia coli and Staphylococcus aureus
Joint Authors
López-Meza, Joel Edmundo
Ochoa-Zarzosa, Alejandra
Guzmán-Rodríguez, Jaquelina Julia
López-Gómez, Rodolfo
Suárez-Rodríguez, Luis M.
Rodríguez-Zapata, Luis C.
Salgado-Garciglia, Rafael
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-12
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Antimicrobial therapy is a useful tool to control infectious diseases in general and rising antibiotic resistant microorganisms in particular.
Alternative strategies are desirable, and antimicrobial peptides (AMP) represent attractive control agents.
Mexican avocado (Persea americana var.
drymifolia) is used in traditional medicine; however, the AMP production has not been reported in this plant.
We obtained a cDNA library from avocado fruit and clone PaDef was identified, which has a cDNA (249 bp) encoding a protein (78 aa) homologous with plant defensins (>80%).
We expressed the defensin PaDef cDNA (pBME3) in the bovine endothelial cell line BVE-E6E7.
Polyclonal and clonal populations were obtained and their activity was evaluated against Escherichia coli, Staphylococcus aureus, and Candida albicans.
E.
coli viability was inhibited with 100 μg/mL of total protein from clones (>55%).
Also, S.
aureus viability was inhibited from 50 μg/mL total protein (27–38%) but was more evident at 100 μg/mL (52–65%).
This inhibition was higher than the effect showed by polyclonal population (~23%).
Finally, we did not detect activity against C.
albicans.
These results are the first report that shows antimicrobial activity of a defensin produced by avocado and suggest that this AMP could be used in the control of pathogens.
American Psychological Association (APA)
Guzmán-Rodríguez, Jaquelina Julia& López-Gómez, Rodolfo& Suárez-Rodríguez, Luis M.& Salgado-Garciglia, Rafael& Rodríguez-Zapata, Luis C.& Ochoa-Zarzosa, Alejandra…[et al.]. 2013. Antibacterial Activity of Defensin PaDef from Avocado Fruit (Persea americana var. drymifolia) Expressed in Endothelial Cells against Escherichia coli and Staphylococcus aureus. BioMed Research International،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1031173
Modern Language Association (MLA)
Guzmán-Rodríguez, Jaquelina Julia…[et al.]. Antibacterial Activity of Defensin PaDef from Avocado Fruit (Persea americana var. drymifolia) Expressed in Endothelial Cells against Escherichia coli and Staphylococcus aureus. BioMed Research International No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1031173
American Medical Association (AMA)
Guzmán-Rodríguez, Jaquelina Julia& López-Gómez, Rodolfo& Suárez-Rodríguez, Luis M.& Salgado-Garciglia, Rafael& Rodríguez-Zapata, Luis C.& Ochoa-Zarzosa, Alejandra…[et al.]. Antibacterial Activity of Defensin PaDef from Avocado Fruit (Persea americana var. drymifolia) Expressed in Endothelial Cells against Escherichia coli and Staphylococcus aureus. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1031173
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1031173