EphB4 Tyrosine Kinase Stimulation Inhibits Growth of MDA-MB-231 Breast Cancer Cells in a Dose and Time Dependent Manner
المؤلفون المشاركون
Barneh, Farnaz
Moshayedi, Mona
Mirmohammadsadeghi, Hamid
Haghjooy-Javanmard, Shaghayegh
Badri, Shirinsadat
Sabzghabaee, A. M.
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-12-23
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Background.
EphB4 receptor tyrosine kinase is of diagnostic and therapeutic value due to its overexpression in breast tumors.
Dual functions of tumor promotion and suppression have been reported for this receptor based on presence or absence of its ligand.
To elucidate such discrepancy, we aimed to determine the effect of time- and dose-dependent stimulation of EphB4 on viability and invasion of breast cancer cells via recombinant ephrinB2-Fc.
Methods.
Cells were seeded into multiwell plates and were stimulated by various concentrations of preclustered ephrinB2-Fc.
Cell viability was measured on days 3 and 6 following treatment using alamar-blue when cells were in different states of confluence.
Results.
Stimulation of cells with ephrinB2 did not pose any significant effect on cell viability before reaching confluence, while inhibition of cell growth was detected after 6 days when cells were in postconfluent state following a dose-dependent manner.
EphrinB2 treatment did not affect tubular formation and invasion on matrigel.
Conclusion.
This study showed that EphB4 can differentially inhibit cells at post confluent state and that presence of ligand manifests growth-inhibitory properties of EphB4 receptor.
It is concluded that growth inhibition has occurred possibly due to long treatment with ligand, a process which leads to receptor downregulation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Barneh, Farnaz& Moshayedi, Mona& Mirmohammadsadeghi, Hamid& Haghjooy-Javanmard, Shaghayegh& Sabzghabaee, A. M.& Badri, Shirinsadat. 2013. EphB4 Tyrosine Kinase Stimulation Inhibits Growth of MDA-MB-231 Breast Cancer Cells in a Dose and Time Dependent Manner. Disease Markers،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1031231
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Barneh, Farnaz…[et al.]. EphB4 Tyrosine Kinase Stimulation Inhibits Growth of MDA-MB-231 Breast Cancer Cells in a Dose and Time Dependent Manner. Disease Markers No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-1031231
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Barneh, Farnaz& Moshayedi, Mona& Mirmohammadsadeghi, Hamid& Haghjooy-Javanmard, Shaghayegh& Sabzghabaee, A. M.& Badri, Shirinsadat. EphB4 Tyrosine Kinase Stimulation Inhibits Growth of MDA-MB-231 Breast Cancer Cells in a Dose and Time Dependent Manner. Disease Markers. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1031231
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1031231
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر