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EphB4 Tyrosine Kinase Stimulation Inhibits Growth of MDA-MB-231 Breast Cancer Cells in a Dose and Time Dependent Manner
Joint Authors
Barneh, Farnaz
Moshayedi, Mona
Mirmohammadsadeghi, Hamid
Haghjooy-Javanmard, Shaghayegh
Badri, Shirinsadat
Sabzghabaee, A. M.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-23
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Background.
EphB4 receptor tyrosine kinase is of diagnostic and therapeutic value due to its overexpression in breast tumors.
Dual functions of tumor promotion and suppression have been reported for this receptor based on presence or absence of its ligand.
To elucidate such discrepancy, we aimed to determine the effect of time- and dose-dependent stimulation of EphB4 on viability and invasion of breast cancer cells via recombinant ephrinB2-Fc.
Methods.
Cells were seeded into multiwell plates and were stimulated by various concentrations of preclustered ephrinB2-Fc.
Cell viability was measured on days 3 and 6 following treatment using alamar-blue when cells were in different states of confluence.
Results.
Stimulation of cells with ephrinB2 did not pose any significant effect on cell viability before reaching confluence, while inhibition of cell growth was detected after 6 days when cells were in postconfluent state following a dose-dependent manner.
EphrinB2 treatment did not affect tubular formation and invasion on matrigel.
Conclusion.
This study showed that EphB4 can differentially inhibit cells at post confluent state and that presence of ligand manifests growth-inhibitory properties of EphB4 receptor.
It is concluded that growth inhibition has occurred possibly due to long treatment with ligand, a process which leads to receptor downregulation.
American Psychological Association (APA)
Barneh, Farnaz& Moshayedi, Mona& Mirmohammadsadeghi, Hamid& Haghjooy-Javanmard, Shaghayegh& Sabzghabaee, A. M.& Badri, Shirinsadat. 2013. EphB4 Tyrosine Kinase Stimulation Inhibits Growth of MDA-MB-231 Breast Cancer Cells in a Dose and Time Dependent Manner. Disease Markers،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1031231
Modern Language Association (MLA)
Barneh, Farnaz…[et al.]. EphB4 Tyrosine Kinase Stimulation Inhibits Growth of MDA-MB-231 Breast Cancer Cells in a Dose and Time Dependent Manner. Disease Markers No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-1031231
American Medical Association (AMA)
Barneh, Farnaz& Moshayedi, Mona& Mirmohammadsadeghi, Hamid& Haghjooy-Javanmard, Shaghayegh& Sabzghabaee, A. M.& Badri, Shirinsadat. EphB4 Tyrosine Kinase Stimulation Inhibits Growth of MDA-MB-231 Breast Cancer Cells in a Dose and Time Dependent Manner. Disease Markers. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1031231
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1031231