Studies of the Interaction between Isoimperatorin and Human Serum Albumin by Multispectroscopic Method: Identification of Possible Binding Site of the Compound Using Esterase Activity of the Protein
المؤلفون المشاركون
Ranjbar, Samira
Ghobadi, Sirous
Bijari, Nooshin
Gholamzadeh, Saeed
Moradi, Nastaran
Ashrafi-Kooshk, Mohammad Reza
Aghaei, Abbas
Khodarahmi, Reza
Shokoohinia, Yalda
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-11-10
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الطب البشري
تكنولوجيا المعلومات وعلم الحاسوب
الملخص EN
Isoimperatorin is one of the main components of Prangos ferulacea as a linear furanocoumarin and used as anti-inflammatory, analgesic, antispasmodic, and anticancer drug.
Human serum albumin (HSA) is a principal extracellular protein with a high concentration in blood plasma and carrier for many drugs to different molecular targets.
Since the carrying of drug by HSA may affect on its structure and action, we decided to investigate the interaction between HSA and isoimperatorin using fluorescence and UV spectroscopy.
Fluorescence data indicated that isoimperatorin quenches the intrinsic fluorescence of the HSA via a static mechanism and hydrophobic interaction play the major role in the drug binding.
The binding average distance between isoimperatorin and Trp 214 of HSA was estimated on the basis of the theory of Förster energy transfer.
Decrease of protein surface hydrophobicity (PSH) was also documented upon isoimperatorin binding.
Furthermore, the synchronous fluorescence spectra show that the microenvironment of the tryptophan residues does not have obvious changes.
Site marker compettive and fluorescence experiments revealed that the binding of isoimperatorin to HSA occurred at or near site I.
Finally, the binding details between isoimperatorin and HSA were further confirmed by molecular docking and esterase activity inhibition studies which revealed that drug was bound at subdomain IIA.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ranjbar, Samira& Shokoohinia, Yalda& Ghobadi, Sirous& Bijari, Nooshin& Gholamzadeh, Saeed& Moradi, Nastaran…[et al.]. 2013. Studies of the Interaction between Isoimperatorin and Human Serum Albumin by Multispectroscopic Method: Identification of Possible Binding Site of the Compound Using Esterase Activity of the Protein. The Scientific World Journal،Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-1032766
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ranjbar, Samira…[et al.]. Studies of the Interaction between Isoimperatorin and Human Serum Albumin by Multispectroscopic Method: Identification of Possible Binding Site of the Compound Using Esterase Activity of the Protein. The Scientific World Journal No. 2013 (2013), pp.1-13.
https://search.emarefa.net/detail/BIM-1032766
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ranjbar, Samira& Shokoohinia, Yalda& Ghobadi, Sirous& Bijari, Nooshin& Gholamzadeh, Saeed& Moradi, Nastaran…[et al.]. Studies of the Interaction between Isoimperatorin and Human Serum Albumin by Multispectroscopic Method: Identification of Possible Binding Site of the Compound Using Esterase Activity of the Protein. The Scientific World Journal. 2013. Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-1032766
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1032766
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر