Studies of the Interaction between Isoimperatorin and Human Serum Albumin by Multispectroscopic Method: Identification of Possible Binding Site of the Compound Using Esterase Activity of the Protein
Joint Authors
Ranjbar, Samira
Ghobadi, Sirous
Bijari, Nooshin
Gholamzadeh, Saeed
Moradi, Nastaran
Ashrafi-Kooshk, Mohammad Reza
Aghaei, Abbas
Khodarahmi, Reza
Shokoohinia, Yalda
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-10
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Isoimperatorin is one of the main components of Prangos ferulacea as a linear furanocoumarin and used as anti-inflammatory, analgesic, antispasmodic, and anticancer drug.
Human serum albumin (HSA) is a principal extracellular protein with a high concentration in blood plasma and carrier for many drugs to different molecular targets.
Since the carrying of drug by HSA may affect on its structure and action, we decided to investigate the interaction between HSA and isoimperatorin using fluorescence and UV spectroscopy.
Fluorescence data indicated that isoimperatorin quenches the intrinsic fluorescence of the HSA via a static mechanism and hydrophobic interaction play the major role in the drug binding.
The binding average distance between isoimperatorin and Trp 214 of HSA was estimated on the basis of the theory of Förster energy transfer.
Decrease of protein surface hydrophobicity (PSH) was also documented upon isoimperatorin binding.
Furthermore, the synchronous fluorescence spectra show that the microenvironment of the tryptophan residues does not have obvious changes.
Site marker compettive and fluorescence experiments revealed that the binding of isoimperatorin to HSA occurred at or near site I.
Finally, the binding details between isoimperatorin and HSA were further confirmed by molecular docking and esterase activity inhibition studies which revealed that drug was bound at subdomain IIA.
American Psychological Association (APA)
Ranjbar, Samira& Shokoohinia, Yalda& Ghobadi, Sirous& Bijari, Nooshin& Gholamzadeh, Saeed& Moradi, Nastaran…[et al.]. 2013. Studies of the Interaction between Isoimperatorin and Human Serum Albumin by Multispectroscopic Method: Identification of Possible Binding Site of the Compound Using Esterase Activity of the Protein. The Scientific World Journal،Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-1032766
Modern Language Association (MLA)
Ranjbar, Samira…[et al.]. Studies of the Interaction between Isoimperatorin and Human Serum Albumin by Multispectroscopic Method: Identification of Possible Binding Site of the Compound Using Esterase Activity of the Protein. The Scientific World Journal No. 2013 (2013), pp.1-13.
https://search.emarefa.net/detail/BIM-1032766
American Medical Association (AMA)
Ranjbar, Samira& Shokoohinia, Yalda& Ghobadi, Sirous& Bijari, Nooshin& Gholamzadeh, Saeed& Moradi, Nastaran…[et al.]. Studies of the Interaction between Isoimperatorin and Human Serum Albumin by Multispectroscopic Method: Identification of Possible Binding Site of the Compound Using Esterase Activity of the Protein. The Scientific World Journal. 2013. Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-1032766
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1032766