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Lipolytic Potential of Aspergillus japonicus LAB01: Production, Partial Purification, and Characterisation of an Extracellular Lipase
المؤلفون المشاركون
Souza, Lívia Tereza Andrade
Oliveira, Jamil S.
dos Santos, Vera L.
Regis, Wiliam C. B.
Resende, Rodrigo R.
Santoro, M. M.
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-10-29
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Lipolytic potential of Aspergillus japonicus LAB01 was investigated by describing the catalytic properties and stability of a secreted extracellular lipase.
Enzyme production was considered high under room temperature after 4 days using sunflower oil and a combination of casein with sodium nitrate.
Lipase was partially purified by 3.9-fold, resulting in a 44.2% yield using ammonium sulphate precipitation (60%) quantified with Superose 12 HR gel filtration chromatography.
The activity of the enzyme was maximised at pH 8.5, and the enzyme demonstrated stability under alkaline conditions.
The optimum temperature was found to be 45°C, and the enzyme was stable for up to 100 minutes, with more than 80% of initial activity remaining after incubation at this temperature.
Partially purified enzyme showed reasonable stability with triton X-100 and was activated in the presence of organic solvents (toluene, hexane, and methanol).
Among the tested ions, only Cu2+, Ni2+, and Al3+ showed inhibitory effects.
Substrate specificity of the lipase was higher for C14 among various p-nitrophenyl esters assayed.
The KM and Vmax values of the purified enzyme for p-nitrophenyl palmitate were 0.13 mM and 12.58 umol/(L·min), respectively.
These features render a novel biocatalyst for industrial applications.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Souza, Lívia Tereza Andrade& Oliveira, Jamil S.& dos Santos, Vera L.& Regis, Wiliam C. B.& Santoro, M. M.& Resende, Rodrigo R.. 2014. Lipolytic Potential of Aspergillus japonicus LAB01: Production, Partial Purification, and Characterisation of an Extracellular Lipase. BioMed Research International،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1034378
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Souza, Lívia Tereza Andrade…[et al.]. Lipolytic Potential of Aspergillus japonicus LAB01: Production, Partial Purification, and Characterisation of an Extracellular Lipase. BioMed Research International No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-1034378
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Souza, Lívia Tereza Andrade& Oliveira, Jamil S.& dos Santos, Vera L.& Regis, Wiliam C. B.& Santoro, M. M.& Resende, Rodrigo R.. Lipolytic Potential of Aspergillus japonicus LAB01: Production, Partial Purification, and Characterisation of an Extracellular Lipase. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1034378
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1034378
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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