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Lipolytic Potential of Aspergillus japonicus LAB01: Production, Partial Purification, and Characterisation of an Extracellular Lipase
Joint Authors
Souza, Lívia Tereza Andrade
Oliveira, Jamil S.
dos Santos, Vera L.
Regis, Wiliam C. B.
Resende, Rodrigo R.
Santoro, M. M.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-10-29
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Lipolytic potential of Aspergillus japonicus LAB01 was investigated by describing the catalytic properties and stability of a secreted extracellular lipase.
Enzyme production was considered high under room temperature after 4 days using sunflower oil and a combination of casein with sodium nitrate.
Lipase was partially purified by 3.9-fold, resulting in a 44.2% yield using ammonium sulphate precipitation (60%) quantified with Superose 12 HR gel filtration chromatography.
The activity of the enzyme was maximised at pH 8.5, and the enzyme demonstrated stability under alkaline conditions.
The optimum temperature was found to be 45°C, and the enzyme was stable for up to 100 minutes, with more than 80% of initial activity remaining after incubation at this temperature.
Partially purified enzyme showed reasonable stability with triton X-100 and was activated in the presence of organic solvents (toluene, hexane, and methanol).
Among the tested ions, only Cu2+, Ni2+, and Al3+ showed inhibitory effects.
Substrate specificity of the lipase was higher for C14 among various p-nitrophenyl esters assayed.
The KM and Vmax values of the purified enzyme for p-nitrophenyl palmitate were 0.13 mM and 12.58 umol/(L·min), respectively.
These features render a novel biocatalyst for industrial applications.
American Psychological Association (APA)
Souza, Lívia Tereza Andrade& Oliveira, Jamil S.& dos Santos, Vera L.& Regis, Wiliam C. B.& Santoro, M. M.& Resende, Rodrigo R.. 2014. Lipolytic Potential of Aspergillus japonicus LAB01: Production, Partial Purification, and Characterisation of an Extracellular Lipase. BioMed Research International،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1034378
Modern Language Association (MLA)
Souza, Lívia Tereza Andrade…[et al.]. Lipolytic Potential of Aspergillus japonicus LAB01: Production, Partial Purification, and Characterisation of an Extracellular Lipase. BioMed Research International No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-1034378
American Medical Association (AMA)
Souza, Lívia Tereza Andrade& Oliveira, Jamil S.& dos Santos, Vera L.& Regis, Wiliam C. B.& Santoro, M. M.& Resende, Rodrigo R.. Lipolytic Potential of Aspergillus japonicus LAB01: Production, Partial Purification, and Characterisation of an Extracellular Lipase. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1034378
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1034378