Freeze-Drying of Plant Tissue Containing HBV Surface Antigen for the Oral Vaccine against Hepatitis B
المؤلفون المشاركون
Czyż, Marcin
Dembczyński, Radosław
Marecik, Roman
Wojas-Turek, Justyna
Milczarek, Magdalena
Pajtasz-Piasecka, Elżbieta
Pniewski, Tomasz
Wietrzyk, Joanna
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-10-12
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
The aim of this study was to develop a freeze-drying protocol facilitating successful processing of plant material containing the small surface antigen of hepatitis B virus (S-HBsAg) while preserving its VLP structure and immunogenicity.
Freeze-drying of the antigen in lettuce leaf tissue, without any isolation or purification step, was investigated.
Each process step was consecutively evaluated and the best parameters were applied.
Several drying profiles and excipients were tested.
The profile of 20°C for 20 h for primary and 22°C for 2 h for secondary drying as well as sucrose expressed efficient stabilisation of S-HBsAg during freeze-drying.
Freezing rate and postprocess residual moisture were also analysed as important factors affecting S-HBsAg preservation.
The process was reproducible and provided a product with VLP content up to 200 µg/g DW.
Assays for VLPs and total antigen together with animal immunisation trials confirmed preservation of antigenicity and immunogenicity of S-HBsAg in freeze-dried powder.
Long-term stability tests revealed that the stored freeze-dried product was stable at 4°C for one year, but degraded at elevated temperatures.
As a result, a basis for an efficient freeze-drying process has been established and a suitable semiproduct for oral plant-derived vaccine against HBV was obtained.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Czyż, Marcin& Dembczyński, Radosław& Marecik, Roman& Wojas-Turek, Justyna& Milczarek, Magdalena& Pajtasz-Piasecka, Elżbieta…[et al.]. 2014. Freeze-Drying of Plant Tissue Containing HBV Surface Antigen for the Oral Vaccine against Hepatitis B. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1034475
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Czyż, Marcin…[et al.]. Freeze-Drying of Plant Tissue Containing HBV Surface Antigen for the Oral Vaccine against Hepatitis B. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1034475
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Czyż, Marcin& Dembczyński, Radosław& Marecik, Roman& Wojas-Turek, Justyna& Milczarek, Magdalena& Pajtasz-Piasecka, Elżbieta…[et al.]. Freeze-Drying of Plant Tissue Containing HBV Surface Antigen for the Oral Vaccine against Hepatitis B. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1034475
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1034475
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر