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Freeze-Drying of Plant Tissue Containing HBV Surface Antigen for the Oral Vaccine against Hepatitis B
Joint Authors
Czyż, Marcin
Dembczyński, Radosław
Marecik, Roman
Wojas-Turek, Justyna
Milczarek, Magdalena
Pajtasz-Piasecka, Elżbieta
Pniewski, Tomasz
Wietrzyk, Joanna
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-10-12
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The aim of this study was to develop a freeze-drying protocol facilitating successful processing of plant material containing the small surface antigen of hepatitis B virus (S-HBsAg) while preserving its VLP structure and immunogenicity.
Freeze-drying of the antigen in lettuce leaf tissue, without any isolation or purification step, was investigated.
Each process step was consecutively evaluated and the best parameters were applied.
Several drying profiles and excipients were tested.
The profile of 20°C for 20 h for primary and 22°C for 2 h for secondary drying as well as sucrose expressed efficient stabilisation of S-HBsAg during freeze-drying.
Freezing rate and postprocess residual moisture were also analysed as important factors affecting S-HBsAg preservation.
The process was reproducible and provided a product with VLP content up to 200 µg/g DW.
Assays for VLPs and total antigen together with animal immunisation trials confirmed preservation of antigenicity and immunogenicity of S-HBsAg in freeze-dried powder.
Long-term stability tests revealed that the stored freeze-dried product was stable at 4°C for one year, but degraded at elevated temperatures.
As a result, a basis for an efficient freeze-drying process has been established and a suitable semiproduct for oral plant-derived vaccine against HBV was obtained.
American Psychological Association (APA)
Czyż, Marcin& Dembczyński, Radosław& Marecik, Roman& Wojas-Turek, Justyna& Milczarek, Magdalena& Pajtasz-Piasecka, Elżbieta…[et al.]. 2014. Freeze-Drying of Plant Tissue Containing HBV Surface Antigen for the Oral Vaccine against Hepatitis B. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1034475
Modern Language Association (MLA)
Czyż, Marcin…[et al.]. Freeze-Drying of Plant Tissue Containing HBV Surface Antigen for the Oral Vaccine against Hepatitis B. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1034475
American Medical Association (AMA)
Czyż, Marcin& Dembczyński, Radosław& Marecik, Roman& Wojas-Turek, Justyna& Milczarek, Magdalena& Pajtasz-Piasecka, Elżbieta…[et al.]. Freeze-Drying of Plant Tissue Containing HBV Surface Antigen for the Oral Vaccine against Hepatitis B. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1034475
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1034475