Molecular Cloning and Biochemical Characterization of a Recombinant Sterol 3-O-Glucosyltransferase from Gymnema sylvestre R.Br. Catalyzing Biosynthesis of Steryl Glucosides
المؤلفون المشاركون
Sangwan, Neelam S.
Tiwari, Pragya
Sangwan, Rajender Singh
Asha, Rajender Singh
Mishra, B. N.
Sabir, Farzana
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-08-27
دولة النشر
مصر
عدد الصفحات
14
التخصصات الرئيسية
الملخص EN
Gymnema sylvestre R.Br., a pharmacologically important herb vernacularly called Gur-Mar (sugar eliminator), is widely known for its antidiabetic action.
This property of the herb has been attributed to the presence of bioactive triterpene glycosides.
Although some information regarding pharmacology and phytochemical profiles of the plant are available, no attempts have been made so far to decipher the biosynthetic pathway and key enzymes involved in biosynthesis of steryl glucosides.
The present report deals with the identification and catalytic characterization of a glucosyltransferase, catalyzing biosynthesis of steryl glycosides.
The full length cDNA (2572 bp) contained an open reading frame of 2106 nucleotides that encoded a 701 amino acid protein, falling into GT-B subfamily of glycosyltransferases.
The GsSGT was expressed in Escherichia coli and biochemical characterization of the recombinant enzyme suggested its key role in the biosynthesis of steryl glucosides with catalytic preference for C-3 hydroxyl group of sterols.
To our knowledge, this pertains to be the first report on cloning and biochemical characterization of a sterol metabolism gene from G.
sylvestre R.Br.
catalyzing glucosylation of a variety of sterols of biological origin from diverse organisms such as bacteria, fungi, and plants.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Tiwari, Pragya& Sangwan, Rajender Singh& Asha, Rajender Singh& Mishra, B. N.& Sabir, Farzana& Sangwan, Neelam S.. 2014. Molecular Cloning and Biochemical Characterization of a Recombinant Sterol 3-O-Glucosyltransferase from Gymnema sylvestre R.Br. Catalyzing Biosynthesis of Steryl Glucosides. BioMed Research International،Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-1034608
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Tiwari, Pragya…[et al.]. Molecular Cloning and Biochemical Characterization of a Recombinant Sterol 3-O-Glucosyltransferase from Gymnema sylvestre R.Br. Catalyzing Biosynthesis of Steryl Glucosides. BioMed Research International No. 2014 (2014), pp.1-14.
https://search.emarefa.net/detail/BIM-1034608
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Tiwari, Pragya& Sangwan, Rajender Singh& Asha, Rajender Singh& Mishra, B. N.& Sabir, Farzana& Sangwan, Neelam S.. Molecular Cloning and Biochemical Characterization of a Recombinant Sterol 3-O-Glucosyltransferase from Gymnema sylvestre R.Br. Catalyzing Biosynthesis of Steryl Glucosides. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-1034608
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1034608
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر