Molecular Cloning and Biochemical Characterization of a Recombinant Sterol 3-O-Glucosyltransferase from Gymnema sylvestre R.Br. Catalyzing Biosynthesis of Steryl Glucosides
Joint Authors
Sangwan, Neelam S.
Tiwari, Pragya
Sangwan, Rajender Singh
Asha, Rajender Singh
Mishra, B. N.
Sabir, Farzana
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-27
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Gymnema sylvestre R.Br., a pharmacologically important herb vernacularly called Gur-Mar (sugar eliminator), is widely known for its antidiabetic action.
This property of the herb has been attributed to the presence of bioactive triterpene glycosides.
Although some information regarding pharmacology and phytochemical profiles of the plant are available, no attempts have been made so far to decipher the biosynthetic pathway and key enzymes involved in biosynthesis of steryl glucosides.
The present report deals with the identification and catalytic characterization of a glucosyltransferase, catalyzing biosynthesis of steryl glycosides.
The full length cDNA (2572 bp) contained an open reading frame of 2106 nucleotides that encoded a 701 amino acid protein, falling into GT-B subfamily of glycosyltransferases.
The GsSGT was expressed in Escherichia coli and biochemical characterization of the recombinant enzyme suggested its key role in the biosynthesis of steryl glucosides with catalytic preference for C-3 hydroxyl group of sterols.
To our knowledge, this pertains to be the first report on cloning and biochemical characterization of a sterol metabolism gene from G.
sylvestre R.Br.
catalyzing glucosylation of a variety of sterols of biological origin from diverse organisms such as bacteria, fungi, and plants.
American Psychological Association (APA)
Tiwari, Pragya& Sangwan, Rajender Singh& Asha, Rajender Singh& Mishra, B. N.& Sabir, Farzana& Sangwan, Neelam S.. 2014. Molecular Cloning and Biochemical Characterization of a Recombinant Sterol 3-O-Glucosyltransferase from Gymnema sylvestre R.Br. Catalyzing Biosynthesis of Steryl Glucosides. BioMed Research International،Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-1034608
Modern Language Association (MLA)
Tiwari, Pragya…[et al.]. Molecular Cloning and Biochemical Characterization of a Recombinant Sterol 3-O-Glucosyltransferase from Gymnema sylvestre R.Br. Catalyzing Biosynthesis of Steryl Glucosides. BioMed Research International No. 2014 (2014), pp.1-14.
https://search.emarefa.net/detail/BIM-1034608
American Medical Association (AMA)
Tiwari, Pragya& Sangwan, Rajender Singh& Asha, Rajender Singh& Mishra, B. N.& Sabir, Farzana& Sangwan, Neelam S.. Molecular Cloning and Biochemical Characterization of a Recombinant Sterol 3-O-Glucosyltransferase from Gymnema sylvestre R.Br. Catalyzing Biosynthesis of Steryl Glucosides. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-1034608
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1034608