Tetramethylpyrazine Protects against Hydrogen Peroxide-Provoked Endothelial Dysfunction in Isolated Rat Aortic Rings: Implications for Antioxidant Therapy of Vascular Diseases
المؤلفون المشاركون
Lau, Chi Wai
Ni, Xiaojia
Wong, Siu Ling
Wong, Chi Ming
Shi, Xiaogeng
Cai, Yefeng
Huang, Yu
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-09-09
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Background and Objectives.
Oxidative stress can initiate endothelial dysfunction and atherosclerosis.
This study evaluated whether tetramethylpyrazine (TMP), the predominant active ingredient in Rhizoma Ligustici Wallichii (chuanxiong), prevents endothelial dysfunction in a rat model of oxidative stress.
Methods.
Isolated rat aortic rings were pretreated with various drugs before the induction of endothelial dysfunction by hydrogen peroxide (H2O2).
Changes in isometric tension were then measured in acetylcholine- (ACh-) relaxed rings.
Endothelial nitric oxide synthase (eNOS) expression was evaluated in the rings by Western blotting, and superoxide anion (O2∙-) content was assessed in primary rat aortic endothelial cells by dihydroethidium- (DHE-) mediated fluorescence microscopy.
Results.
ACh-induced endothelium-dependent relaxation (EDR) was disrupted by H2O2 in endothelium-intact aortic rings.
H2O2-impaired relaxation was ameliorated by acute pretreatment with low concentrations of TMP, as well as by pretreatment with catalase and the NADPH oxidase inhibitors, apocynin and diphenyleneiodonium (DPI).
TMP, apocynin, and DPI also reduced O2∙- accumulation in endothelial cells,but TMP failed to alter eNOS expression in aortic rings incubated with H2O2.
Conclusions.
TMP safeguards against oxidative stress-induced endothelial dysfunction, suggesting that the agent might find therapeutic utility in the management of vascular diseases.
However, TMP’s role in inhibiting NADPH oxidase and its vascular-protective mechanism of action requires further investigation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ni, Xiaojia& Wong, Siu Ling& Wong, Chi Ming& Lau, Chi Wai& Shi, Xiaogeng& Cai, Yefeng…[et al.]. 2014. Tetramethylpyrazine Protects against Hydrogen Peroxide-Provoked Endothelial Dysfunction in Isolated Rat Aortic Rings: Implications for Antioxidant Therapy of Vascular Diseases. Evidence-Based Complementary and Alternative Medicine،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1035310
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ni, Xiaojia…[et al.]. Tetramethylpyrazine Protects against Hydrogen Peroxide-Provoked Endothelial Dysfunction in Isolated Rat Aortic Rings: Implications for Antioxidant Therapy of Vascular Diseases. Evidence-Based Complementary and Alternative Medicine No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1035310
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ni, Xiaojia& Wong, Siu Ling& Wong, Chi Ming& Lau, Chi Wai& Shi, Xiaogeng& Cai, Yefeng…[et al.]. Tetramethylpyrazine Protects against Hydrogen Peroxide-Provoked Endothelial Dysfunction in Isolated Rat Aortic Rings: Implications for Antioxidant Therapy of Vascular Diseases. Evidence-Based Complementary and Alternative Medicine. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1035310
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1035310
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر