Tetramethylpyrazine Protects against Hydrogen Peroxide-Provoked Endothelial Dysfunction in Isolated Rat Aortic Rings: Implications for Antioxidant Therapy of Vascular Diseases
Joint Authors
Lau, Chi Wai
Ni, Xiaojia
Wong, Siu Ling
Wong, Chi Ming
Shi, Xiaogeng
Cai, Yefeng
Huang, Yu
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-09-09
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Background and Objectives.
Oxidative stress can initiate endothelial dysfunction and atherosclerosis.
This study evaluated whether tetramethylpyrazine (TMP), the predominant active ingredient in Rhizoma Ligustici Wallichii (chuanxiong), prevents endothelial dysfunction in a rat model of oxidative stress.
Methods.
Isolated rat aortic rings were pretreated with various drugs before the induction of endothelial dysfunction by hydrogen peroxide (H2O2).
Changes in isometric tension were then measured in acetylcholine- (ACh-) relaxed rings.
Endothelial nitric oxide synthase (eNOS) expression was evaluated in the rings by Western blotting, and superoxide anion (O2∙-) content was assessed in primary rat aortic endothelial cells by dihydroethidium- (DHE-) mediated fluorescence microscopy.
Results.
ACh-induced endothelium-dependent relaxation (EDR) was disrupted by H2O2 in endothelium-intact aortic rings.
H2O2-impaired relaxation was ameliorated by acute pretreatment with low concentrations of TMP, as well as by pretreatment with catalase and the NADPH oxidase inhibitors, apocynin and diphenyleneiodonium (DPI).
TMP, apocynin, and DPI also reduced O2∙- accumulation in endothelial cells,but TMP failed to alter eNOS expression in aortic rings incubated with H2O2.
Conclusions.
TMP safeguards against oxidative stress-induced endothelial dysfunction, suggesting that the agent might find therapeutic utility in the management of vascular diseases.
However, TMP’s role in inhibiting NADPH oxidase and its vascular-protective mechanism of action requires further investigation.
American Psychological Association (APA)
Ni, Xiaojia& Wong, Siu Ling& Wong, Chi Ming& Lau, Chi Wai& Shi, Xiaogeng& Cai, Yefeng…[et al.]. 2014. Tetramethylpyrazine Protects against Hydrogen Peroxide-Provoked Endothelial Dysfunction in Isolated Rat Aortic Rings: Implications for Antioxidant Therapy of Vascular Diseases. Evidence-Based Complementary and Alternative Medicine،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1035310
Modern Language Association (MLA)
Ni, Xiaojia…[et al.]. Tetramethylpyrazine Protects against Hydrogen Peroxide-Provoked Endothelial Dysfunction in Isolated Rat Aortic Rings: Implications for Antioxidant Therapy of Vascular Diseases. Evidence-Based Complementary and Alternative Medicine No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1035310
American Medical Association (AMA)
Ni, Xiaojia& Wong, Siu Ling& Wong, Chi Ming& Lau, Chi Wai& Shi, Xiaogeng& Cai, Yefeng…[et al.]. Tetramethylpyrazine Protects against Hydrogen Peroxide-Provoked Endothelial Dysfunction in Isolated Rat Aortic Rings: Implications for Antioxidant Therapy of Vascular Diseases. Evidence-Based Complementary and Alternative Medicine. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1035310
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1035310