Resveratrol Prevention of Diabetic Nephropathy is Associated with the Suppression of Renal Inflammation and Mesangial Cell Proliferation: Possible Roles of AktNF- κ B Pathway

الملخص EN

The present study was to investigate the protection of resveratrol (RSV) in diabetes associated with kidney inflammation and cell proliferation.

Rat mesangial cell and streptozotocin-induced type 1 diabetes mouse model were used.

In vitro, RSV attenuated high glucose-induced plasminogen activator inhibitor (PAI-1) expression and mesangial cell proliferation, as well as Akt and nuclear factor-kappa B (NF-κB) activation.

The similar results were recaptured in the experiment with Akt inhibitors.

In vivo, mice were divided into three groups: control group, diabetes mellitus (DM) group, and RSV-treated DM group.

Compared with control group, the kidney weight to body weight ratio and albumin to creatinine ratio were increased in DM group, but not in RSV-treated DM group.

Furthermore, the increased expression of PAI-1 and intercellular adhesion molecule-1 in diabetic renal cortex were also reduced by RSV administration.

Besides, the kidney p-Akt/Akt ratio and NF-κB were significantly increased in DM group; however, these changes were reversed in RSV-treated DM group.

Additionally, immunohistochemistry results indicated that RSV treatment reduced the density of proliferating cell nuclear antigen-positive cells significantly in glomeruli of diabetic mice.

These results suggest that RSV prevents diabetes-induced renal inflammation and mesangial cell proliferation possibly through Akt/NF-κB pathway inhibition.