Stability of miR-126 in Urine and Its Potential as a Biomarker for Renal Endothelial Injury with Diabetic Nephropathy
المؤلفون المشاركون
Liu, Yang
Gao, Guangqiang
Yang, Chun
Zhou, Kun
Shen, Baozhong
Liang, Hongyan
Jiang, Xiaofeng
المصدر
International Journal of Endocrinology
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-04-17
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Background.
The purpose of the present study was to assess the feasibility of using miR-126 in the urine as a biomarker for diabetic nephropathy.
Methods.
miRNAs were extracted from the urine samples of T2DM patients with diabetic nephropathy (DN; n = 92 ), T2DM without DN ( n = 86 ), and 85 healthy volunteers using quantitative reverse transcriptase polymerase chain reaction (real-time polymerase chain reaction) analysis.
Stability of urinary miR-126 and factors that affected the stability were assessed.
A subgroup analysis was also carried out to compare the urinary miR-126 level in T2DM patients well controlled by the treatment versus those who were not well controlled.
Results.
Urinary miR-126 was stable when the urine samples were kept at room temperature for extended period of time, 4°C, −20°C, and −80°C for up to 12 hours or subjected to 10 freeze-and-thaw cycle.
Urinary miR-126 was significantly higher in T2DM patients with DN ( 5.7 6 ± 0.3 3 versus 3.2 5 ± 0.4 5 in T2DM patients without DN).
Successful treatment significantly reduced urinary miR-126 in T2DM patients with DN to 3.89 ± 0.52 ( P < 0.05 ).
Conclusion.
miR-126 in the urine is stable and it could be used as a biomarker of DN and to monitor the treatment response.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Liu, Yang& Gao, Guangqiang& Yang, Chun& Zhou, Kun& Shen, Baozhong& Liang, Hongyan…[et al.]. 2014. Stability of miR-126 in Urine and Its Potential as a Biomarker for Renal Endothelial Injury with Diabetic Nephropathy. International Journal of Endocrinology،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1036487
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Liu, Yang…[et al.]. Stability of miR-126 in Urine and Its Potential as a Biomarker for Renal Endothelial Injury with Diabetic Nephropathy. International Journal of Endocrinology No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-1036487
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Liu, Yang& Gao, Guangqiang& Yang, Chun& Zhou, Kun& Shen, Baozhong& Liang, Hongyan…[et al.]. Stability of miR-126 in Urine and Its Potential as a Biomarker for Renal Endothelial Injury with Diabetic Nephropathy. International Journal of Endocrinology. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1036487
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1036487
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر