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Stability of miR-126 in Urine and Its Potential as a Biomarker for Renal Endothelial Injury with Diabetic Nephropathy
Joint Authors
Liu, Yang
Gao, Guangqiang
Yang, Chun
Zhou, Kun
Shen, Baozhong
Liang, Hongyan
Jiang, Xiaofeng
Source
International Journal of Endocrinology
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-17
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Background.
The purpose of the present study was to assess the feasibility of using miR-126 in the urine as a biomarker for diabetic nephropathy.
Methods.
miRNAs were extracted from the urine samples of T2DM patients with diabetic nephropathy (DN; n = 92 ), T2DM without DN ( n = 86 ), and 85 healthy volunteers using quantitative reverse transcriptase polymerase chain reaction (real-time polymerase chain reaction) analysis.
Stability of urinary miR-126 and factors that affected the stability were assessed.
A subgroup analysis was also carried out to compare the urinary miR-126 level in T2DM patients well controlled by the treatment versus those who were not well controlled.
Results.
Urinary miR-126 was stable when the urine samples were kept at room temperature for extended period of time, 4°C, −20°C, and −80°C for up to 12 hours or subjected to 10 freeze-and-thaw cycle.
Urinary miR-126 was significantly higher in T2DM patients with DN ( 5.7 6 ± 0.3 3 versus 3.2 5 ± 0.4 5 in T2DM patients without DN).
Successful treatment significantly reduced urinary miR-126 in T2DM patients with DN to 3.89 ± 0.52 ( P < 0.05 ).
Conclusion.
miR-126 in the urine is stable and it could be used as a biomarker of DN and to monitor the treatment response.
American Psychological Association (APA)
Liu, Yang& Gao, Guangqiang& Yang, Chun& Zhou, Kun& Shen, Baozhong& Liang, Hongyan…[et al.]. 2014. Stability of miR-126 in Urine and Its Potential as a Biomarker for Renal Endothelial Injury with Diabetic Nephropathy. International Journal of Endocrinology،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1036487
Modern Language Association (MLA)
Liu, Yang…[et al.]. Stability of miR-126 in Urine and Its Potential as a Biomarker for Renal Endothelial Injury with Diabetic Nephropathy. International Journal of Endocrinology No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-1036487
American Medical Association (AMA)
Liu, Yang& Gao, Guangqiang& Yang, Chun& Zhou, Kun& Shen, Baozhong& Liang, Hongyan…[et al.]. Stability of miR-126 in Urine and Its Potential as a Biomarker for Renal Endothelial Injury with Diabetic Nephropathy. International Journal of Endocrinology. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1036487
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1036487