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Autoregulatory Feedback Mechanism of P38MAPKCaspase-8 in Photodynamic Therapy-HydrophilicLipophilic Tetra-α-(4-carboxyphenoxy) Phthalocyanine Zinc-Induced Apoptosis of Human Hepatocellular Carcinoma Bel-7402 Cells
المؤلفون المشاركون
Wang, Yu
Xia, Chunhui
Chen, Wei
Chen, Yuhang
Wang, Yiyi
Li, Tao
المصدر
International Journal of Photoenergy
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-03-31
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Photodynamic therapy (PDT) is a novel and promising antitumor treatment.
Our previous study showed that hydrophilic/lipophilic tetra-α-(4-carboxyphenoxy) phthalocyanine zinc- (TαPcZn-) mediated PDT (TαPcZn-PDT) inhibits the proliferation of human hepatocellular carcinoma Bel-7402 cells by triggering apoptosis and arresting cell cycle.
However, mechanisms of TαPcZn-PDT-induced apoptosis of Bel-7402 cells have not been fully clarified.
In the present study, therefore, effect of TαPcZn-PDT on apoptosis, P38MAPK, p-P38MAPK, Caspase-8, Caspase-3, Bcl-2, Bid, Cytochrome c, and mitochondria membrane potential in Bel-7402 cells without or with P38MAPK inhibitor SB203580 or Caspase-8 inhibitor Ac-IEFD-CHO was investigated by haematoxylin and eosin (HE) staining assay, flow cytometry analysis of annexin V-FITC/propidium iodide (PI) double staining cells and 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine iodide (JC-1), and immunoblot assay.
We found that TαPcZn-PDT resulted in apoptosis induction, activation of P38MAPK, Caspase-8, Caspase-3, and Bid, downregulation of Bcl-2, release of Cytochrome c from mitochondria, and disruption of mitochondrial membrane potential in TαPcZn-PDT-treated Bel-7402 cells.
In contrast, SB203580 or Ac-IEFD-CHO attenuated induction of apoptosis, activation of P38MAPK, Caspase-8, Caspase-3, and Bid, downregulation of Bcl-2, release of Cytochrome c from mitochondria, and disruption of mitochondrial membrane potential in TαPcZn-PDT-treated Bel-7402 cells.
Taken together, we conclude that Caspase-3, Bcl-2, Bid, and mitochondria are involved in autoregulatory feedback of P38MAPK/Caspase-8 during TαPcZn-PDT-induced apoptosis of Bel-7402 cells.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wang, Yu& Xia, Chunhui& Chen, Wei& Chen, Yuhang& Wang, Yiyi& Li, Tao. 2014. Autoregulatory Feedback Mechanism of P38MAPKCaspase-8 in Photodynamic Therapy-HydrophilicLipophilic Tetra-α-(4-carboxyphenoxy) Phthalocyanine Zinc-Induced Apoptosis of Human Hepatocellular Carcinoma Bel-7402 Cells. International Journal of Photoenergy،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1036790
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wang, Yu…[et al.]. Autoregulatory Feedback Mechanism of P38MAPKCaspase-8 in Photodynamic Therapy-HydrophilicLipophilic Tetra-α-(4-carboxyphenoxy) Phthalocyanine Zinc-Induced Apoptosis of Human Hepatocellular Carcinoma Bel-7402 Cells. International Journal of Photoenergy No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1036790
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wang, Yu& Xia, Chunhui& Chen, Wei& Chen, Yuhang& Wang, Yiyi& Li, Tao. Autoregulatory Feedback Mechanism of P38MAPKCaspase-8 in Photodynamic Therapy-HydrophilicLipophilic Tetra-α-(4-carboxyphenoxy) Phthalocyanine Zinc-Induced Apoptosis of Human Hepatocellular Carcinoma Bel-7402 Cells. International Journal of Photoenergy. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1036790
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1036790
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
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