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Autoregulatory Feedback Mechanism of P38MAPKCaspase-8 in Photodynamic Therapy-HydrophilicLipophilic Tetra-α-(4-carboxyphenoxy) Phthalocyanine Zinc-Induced Apoptosis of Human Hepatocellular Carcinoma Bel-7402 Cells
Joint Authors
Wang, Yu
Xia, Chunhui
Chen, Wei
Chen, Yuhang
Wang, Yiyi
Li, Tao
Source
International Journal of Photoenergy
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-31
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Photodynamic therapy (PDT) is a novel and promising antitumor treatment.
Our previous study showed that hydrophilic/lipophilic tetra-α-(4-carboxyphenoxy) phthalocyanine zinc- (TαPcZn-) mediated PDT (TαPcZn-PDT) inhibits the proliferation of human hepatocellular carcinoma Bel-7402 cells by triggering apoptosis and arresting cell cycle.
However, mechanisms of TαPcZn-PDT-induced apoptosis of Bel-7402 cells have not been fully clarified.
In the present study, therefore, effect of TαPcZn-PDT on apoptosis, P38MAPK, p-P38MAPK, Caspase-8, Caspase-3, Bcl-2, Bid, Cytochrome c, and mitochondria membrane potential in Bel-7402 cells without or with P38MAPK inhibitor SB203580 or Caspase-8 inhibitor Ac-IEFD-CHO was investigated by haematoxylin and eosin (HE) staining assay, flow cytometry analysis of annexin V-FITC/propidium iodide (PI) double staining cells and 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine iodide (JC-1), and immunoblot assay.
We found that TαPcZn-PDT resulted in apoptosis induction, activation of P38MAPK, Caspase-8, Caspase-3, and Bid, downregulation of Bcl-2, release of Cytochrome c from mitochondria, and disruption of mitochondrial membrane potential in TαPcZn-PDT-treated Bel-7402 cells.
In contrast, SB203580 or Ac-IEFD-CHO attenuated induction of apoptosis, activation of P38MAPK, Caspase-8, Caspase-3, and Bid, downregulation of Bcl-2, release of Cytochrome c from mitochondria, and disruption of mitochondrial membrane potential in TαPcZn-PDT-treated Bel-7402 cells.
Taken together, we conclude that Caspase-3, Bcl-2, Bid, and mitochondria are involved in autoregulatory feedback of P38MAPK/Caspase-8 during TαPcZn-PDT-induced apoptosis of Bel-7402 cells.
American Psychological Association (APA)
Wang, Yu& Xia, Chunhui& Chen, Wei& Chen, Yuhang& Wang, Yiyi& Li, Tao. 2014. Autoregulatory Feedback Mechanism of P38MAPKCaspase-8 in Photodynamic Therapy-HydrophilicLipophilic Tetra-α-(4-carboxyphenoxy) Phthalocyanine Zinc-Induced Apoptosis of Human Hepatocellular Carcinoma Bel-7402 Cells. International Journal of Photoenergy،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1036790
Modern Language Association (MLA)
Wang, Yu…[et al.]. Autoregulatory Feedback Mechanism of P38MAPKCaspase-8 in Photodynamic Therapy-HydrophilicLipophilic Tetra-α-(4-carboxyphenoxy) Phthalocyanine Zinc-Induced Apoptosis of Human Hepatocellular Carcinoma Bel-7402 Cells. International Journal of Photoenergy No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1036790
American Medical Association (AMA)
Wang, Yu& Xia, Chunhui& Chen, Wei& Chen, Yuhang& Wang, Yiyi& Li, Tao. Autoregulatory Feedback Mechanism of P38MAPKCaspase-8 in Photodynamic Therapy-HydrophilicLipophilic Tetra-α-(4-carboxyphenoxy) Phthalocyanine Zinc-Induced Apoptosis of Human Hepatocellular Carcinoma Bel-7402 Cells. International Journal of Photoenergy. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1036790
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1036790