Effect of Light-Activated Hypocrellin B on the Growth and Membrane Permeability of Gram-Negative Escherichia coli Cells
المؤلفون المشاركون
Jiang, Yuan
Tang, Qingjuan
Leung, Albert Wingnang
Zhang, Hongwei
Xu, Chuanshan
المصدر
International Journal of Photoenergy
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-05-13
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Aim.
To investigate the effect of light-activated hypocrellin B on the growth and membrane permeability of Gram-negative bacteria.
Methods.
Escherichia coli (E.
coli) as a model bacterium of Gram-negative bacteria was incubated with various concentrations of hypocrellin B for 60 min and was subsequently irradiated by blue light with wavelength of 470 nm at the dose of 12 J/cm2.
Colony forming units were counted and the growth inhibition rate of E.
coli cells was calculated after light-activated hypocrellin B.
Membrane permeability was measured using flow cytometry and confocal laser scanning microscopy (CLSM) with propidium iodide (PI) staining.
Bacterial morphology was observed using transmission electron microscopy (TEM).
Reactive oxygen species in bacterial cells were measured using flow cytometry with DCFH-DA staining.
Results.
Significant growth inhibition rate of E.
coli cells was observed after photodynamic action of hypocrellin B.
Remarkable damage to the ultrastructure of E.
coli was also observed by TEM.
Flow cytometry and CLSM observation showed that light-activated hypocrellin B markedly increased membrane permeability of E.
coli.
Flow cytometry showed the intracellular ROS increase in E.
coli treated by photodynamic action of hypocrellin B.
Conclusion.
Light-activated hypocrellin B caused intracellular ROS increase and structural damages and inhibited the growth of Gram-negative E.
coli cells.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Jiang, Yuan& Leung, Albert Wingnang& Tang, Qingjuan& Zhang, Hongwei& Xu, Chuanshan. 2014. Effect of Light-Activated Hypocrellin B on the Growth and Membrane Permeability of Gram-Negative Escherichia coli Cells. International Journal of Photoenergy،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1037147
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Jiang, Yuan…[et al.]. Effect of Light-Activated Hypocrellin B on the Growth and Membrane Permeability of Gram-Negative Escherichia coli Cells. International Journal of Photoenergy No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-1037147
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Jiang, Yuan& Leung, Albert Wingnang& Tang, Qingjuan& Zhang, Hongwei& Xu, Chuanshan. Effect of Light-Activated Hypocrellin B on the Growth and Membrane Permeability of Gram-Negative Escherichia coli Cells. International Journal of Photoenergy. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1037147
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1037147
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر