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Effect of Light-Activated Hypocrellin B on the Growth and Membrane Permeability of Gram-Negative Escherichia coli Cells
Joint Authors
Jiang, Yuan
Tang, Qingjuan
Leung, Albert Wingnang
Zhang, Hongwei
Xu, Chuanshan
Source
International Journal of Photoenergy
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-13
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Aim.
To investigate the effect of light-activated hypocrellin B on the growth and membrane permeability of Gram-negative bacteria.
Methods.
Escherichia coli (E.
coli) as a model bacterium of Gram-negative bacteria was incubated with various concentrations of hypocrellin B for 60 min and was subsequently irradiated by blue light with wavelength of 470 nm at the dose of 12 J/cm2.
Colony forming units were counted and the growth inhibition rate of E.
coli cells was calculated after light-activated hypocrellin B.
Membrane permeability was measured using flow cytometry and confocal laser scanning microscopy (CLSM) with propidium iodide (PI) staining.
Bacterial morphology was observed using transmission electron microscopy (TEM).
Reactive oxygen species in bacterial cells were measured using flow cytometry with DCFH-DA staining.
Results.
Significant growth inhibition rate of E.
coli cells was observed after photodynamic action of hypocrellin B.
Remarkable damage to the ultrastructure of E.
coli was also observed by TEM.
Flow cytometry and CLSM observation showed that light-activated hypocrellin B markedly increased membrane permeability of E.
coli.
Flow cytometry showed the intracellular ROS increase in E.
coli treated by photodynamic action of hypocrellin B.
Conclusion.
Light-activated hypocrellin B caused intracellular ROS increase and structural damages and inhibited the growth of Gram-negative E.
coli cells.
American Psychological Association (APA)
Jiang, Yuan& Leung, Albert Wingnang& Tang, Qingjuan& Zhang, Hongwei& Xu, Chuanshan. 2014. Effect of Light-Activated Hypocrellin B on the Growth and Membrane Permeability of Gram-Negative Escherichia coli Cells. International Journal of Photoenergy،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1037147
Modern Language Association (MLA)
Jiang, Yuan…[et al.]. Effect of Light-Activated Hypocrellin B on the Growth and Membrane Permeability of Gram-Negative Escherichia coli Cells. International Journal of Photoenergy No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-1037147
American Medical Association (AMA)
Jiang, Yuan& Leung, Albert Wingnang& Tang, Qingjuan& Zhang, Hongwei& Xu, Chuanshan. Effect of Light-Activated Hypocrellin B on the Growth and Membrane Permeability of Gram-Negative Escherichia coli Cells. International Journal of Photoenergy. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1037147
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1037147