The Cytotoxic Role of Intermittent High Glucose on Apoptosis and Cell Viability in Pancreatic Beta Cells
المؤلفون المشاركون
Zhang, Zhen
Li, Jing
Yang, Lei
Chen, Rongping
Yang, Rui
Zhang, Hua
Cai, Dehong
Chen, Hong
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-03-17
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Objectives.
Glucose fluctuations are both strong predictor of diabetic complications and crucial factor for beta cell damages.
Here we investigated the effect of intermittent high glucose (IHG) on both cell apoptosis and proliferation activity in INS-1 cells and the potential mechanisms.
Methods.
Cells were treated with normal glucose (5.5 mmol/L), constant high glucose (CHG) (25 mmol/L), and IHG (rotation per 24 h in 11.1 or 25 mmol/L) for 7 days.
Reactive oxygen species (ROS), xanthine oxidase (XOD) level, apoptosis, cell viability, cell cycle, and expression of cyclinD1, p21, p27, and Skp2 were determined.
Results.
We found that IHG induced more significant apoptosis than CHG and normal glucose; intracellular ROS and XOD levels were more markedly increased in cells exposed to IHG.
Cells treated with IHG showed significant decreased cell viability and increased cell proportion in G0/G1 phase.
Cell cycle related proteins such as cyclinD1 and Skp2 were decreased significantly, but expressions of p27 and p21 were increased markedly.
Conclusions.
This study suggested that IHG plays a more toxic effect including both apoptosis-inducing and antiproliferative effects on INS-1 cells.
Excessive activation of cellular stress and regulation of cyclins might be potential mechanism of impairment in INS-1 cells induced by IHG.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhang, Zhen& Li, Jing& Yang, Lei& Chen, Rongping& Yang, Rui& Zhang, Hua…[et al.]. 2014. The Cytotoxic Role of Intermittent High Glucose on Apoptosis and Cell Viability in Pancreatic Beta Cells. Journal of Diabetes Research،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1040334
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhang, Zhen…[et al.]. The Cytotoxic Role of Intermittent High Glucose on Apoptosis and Cell Viability in Pancreatic Beta Cells. Journal of Diabetes Research No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1040334
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhang, Zhen& Li, Jing& Yang, Lei& Chen, Rongping& Yang, Rui& Zhang, Hua…[et al.]. The Cytotoxic Role of Intermittent High Glucose on Apoptosis and Cell Viability in Pancreatic Beta Cells. Journal of Diabetes Research. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1040334
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1040334
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر