![](/images/graphics-bg.png)
The Cytotoxic Role of Intermittent High Glucose on Apoptosis and Cell Viability in Pancreatic Beta Cells
Joint Authors
Zhang, Zhen
Li, Jing
Yang, Lei
Chen, Rongping
Yang, Rui
Zhang, Hua
Cai, Dehong
Chen, Hong
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-17
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Objectives.
Glucose fluctuations are both strong predictor of diabetic complications and crucial factor for beta cell damages.
Here we investigated the effect of intermittent high glucose (IHG) on both cell apoptosis and proliferation activity in INS-1 cells and the potential mechanisms.
Methods.
Cells were treated with normal glucose (5.5 mmol/L), constant high glucose (CHG) (25 mmol/L), and IHG (rotation per 24 h in 11.1 or 25 mmol/L) for 7 days.
Reactive oxygen species (ROS), xanthine oxidase (XOD) level, apoptosis, cell viability, cell cycle, and expression of cyclinD1, p21, p27, and Skp2 were determined.
Results.
We found that IHG induced more significant apoptosis than CHG and normal glucose; intracellular ROS and XOD levels were more markedly increased in cells exposed to IHG.
Cells treated with IHG showed significant decreased cell viability and increased cell proportion in G0/G1 phase.
Cell cycle related proteins such as cyclinD1 and Skp2 were decreased significantly, but expressions of p27 and p21 were increased markedly.
Conclusions.
This study suggested that IHG plays a more toxic effect including both apoptosis-inducing and antiproliferative effects on INS-1 cells.
Excessive activation of cellular stress and regulation of cyclins might be potential mechanism of impairment in INS-1 cells induced by IHG.
American Psychological Association (APA)
Zhang, Zhen& Li, Jing& Yang, Lei& Chen, Rongping& Yang, Rui& Zhang, Hua…[et al.]. 2014. The Cytotoxic Role of Intermittent High Glucose on Apoptosis and Cell Viability in Pancreatic Beta Cells. Journal of Diabetes Research،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1040334
Modern Language Association (MLA)
Zhang, Zhen…[et al.]. The Cytotoxic Role of Intermittent High Glucose on Apoptosis and Cell Viability in Pancreatic Beta Cells. Journal of Diabetes Research No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1040334
American Medical Association (AMA)
Zhang, Zhen& Li, Jing& Yang, Lei& Chen, Rongping& Yang, Rui& Zhang, Hua…[et al.]. The Cytotoxic Role of Intermittent High Glucose on Apoptosis and Cell Viability in Pancreatic Beta Cells. Journal of Diabetes Research. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1040334
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1040334