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P38 Plays an Important Role in Glucolipotoxicity-Induced Apoptosis in INS-1 Cells
المؤلفون المشاركون
Cai, Xiaoling
Ji, Linong
Han, Xueyao
Zhou, Lingli
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-03-05
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Objectives.
The mechanism underlying the regulation of glucolipotoxicity-induced apoptosis by MAPKs was examined in INS-1 cells.
Methods.
The rat insulinoma cell line INS-1 was cotreated with glucose (30 mM) and palmitic acid (0.2 mM) (GLU+PA).
Apoptosis was assessed by cell morphology and detection of PARP cleavage.
The activation of MAPKs was examined by Western blotting using specific antibodies against the phosphorylated forms of JNK, ERK1/2, and P38.
Results.
(1) Live cell imaging studies showed that treatment with GLU+PA for 72 h induced significant cell death, concomitant with PARP-1 cleavage and caspase-3 activation, which peaked at 96 h of treatment.
(2) Western blot analysis of the activation of MAPKs during GLU+PA-induced INS-1 cell apoptosis showed that phosphorylation of P38 increased gradually and reached a peak at 96 h, which coincided with PARP-1 cleavage.
A transient increase of ERK activation was followed by a rapid decline at 96 h, whereas JNK phosphorylation status remained unchanged in response to GLU+PA.
(3) Phosphorylation of insulin receptor substrate (IRS)-2 at 48 h of treatment triggered its degradation, which coincided with P38 activation.
(4) Inhibition of P38, but not JNK or ERK, blocked GLU+PA-induced INS-1 cell apoptosis.
Conclusions.
P38 may be involved in the regulation of glucolipotoxicity-induced apoptosis through the phosphorylation of IRS-2.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhou, Lingli& Cai, Xiaoling& Han, Xueyao& Ji, Linong. 2014. P38 Plays an Important Role in Glucolipotoxicity-Induced Apoptosis in INS-1 Cells. Journal of Diabetes Research،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1040382
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhou, Lingli…[et al.]. P38 Plays an Important Role in Glucolipotoxicity-Induced Apoptosis in INS-1 Cells. Journal of Diabetes Research No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-1040382
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhou, Lingli& Cai, Xiaoling& Han, Xueyao& Ji, Linong. P38 Plays an Important Role in Glucolipotoxicity-Induced Apoptosis in INS-1 Cells. Journal of Diabetes Research. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1040382
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1040382
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