P38 Plays an Important Role in Glucolipotoxicity-Induced Apoptosis in INS-1 Cells
Joint Authors
Cai, Xiaoling
Ji, Linong
Han, Xueyao
Zhou, Lingli
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-05
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Objectives.
The mechanism underlying the regulation of glucolipotoxicity-induced apoptosis by MAPKs was examined in INS-1 cells.
Methods.
The rat insulinoma cell line INS-1 was cotreated with glucose (30 mM) and palmitic acid (0.2 mM) (GLU+PA).
Apoptosis was assessed by cell morphology and detection of PARP cleavage.
The activation of MAPKs was examined by Western blotting using specific antibodies against the phosphorylated forms of JNK, ERK1/2, and P38.
Results.
(1) Live cell imaging studies showed that treatment with GLU+PA for 72 h induced significant cell death, concomitant with PARP-1 cleavage and caspase-3 activation, which peaked at 96 h of treatment.
(2) Western blot analysis of the activation of MAPKs during GLU+PA-induced INS-1 cell apoptosis showed that phosphorylation of P38 increased gradually and reached a peak at 96 h, which coincided with PARP-1 cleavage.
A transient increase of ERK activation was followed by a rapid decline at 96 h, whereas JNK phosphorylation status remained unchanged in response to GLU+PA.
(3) Phosphorylation of insulin receptor substrate (IRS)-2 at 48 h of treatment triggered its degradation, which coincided with P38 activation.
(4) Inhibition of P38, but not JNK or ERK, blocked GLU+PA-induced INS-1 cell apoptosis.
Conclusions.
P38 may be involved in the regulation of glucolipotoxicity-induced apoptosis through the phosphorylation of IRS-2.
American Psychological Association (APA)
Zhou, Lingli& Cai, Xiaoling& Han, Xueyao& Ji, Linong. 2014. P38 Plays an Important Role in Glucolipotoxicity-Induced Apoptosis in INS-1 Cells. Journal of Diabetes Research،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1040382
Modern Language Association (MLA)
Zhou, Lingli…[et al.]. P38 Plays an Important Role in Glucolipotoxicity-Induced Apoptosis in INS-1 Cells. Journal of Diabetes Research No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-1040382
American Medical Association (AMA)
Zhou, Lingli& Cai, Xiaoling& Han, Xueyao& Ji, Linong. P38 Plays an Important Role in Glucolipotoxicity-Induced Apoptosis in INS-1 Cells. Journal of Diabetes Research. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1040382
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1040382