Dose of Incorporated Immunodominant Antigen in Recombinant BCG Impacts Modestly on Th1 Immune Response and Protective Efficiency against Mycobacterium tuberculosis in Mice
المؤلفون المشاركون
Wu, Kang
Fan, Xiao-Yong
Ma, Hui
Liu, Fang
Yang, Hua
Kang, Han
Chen, Ning-Ning
Yuan, Qin
Zhou, Wen-Jiang
المصدر
Journal of Immunology Research
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-07-23
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
One approach for improving BCG efficacy is to utilize BCG as vehicle to develop recombinant BCG (rBCG) strains overexpressing Mycobacterium tuberculosis (M.
tb) antigens.
Also expression level of a candidate antigen should impact the final T cell responses conferred by rBCG.
In this study, based on our previously constructed differential expression system, we developed two rBCG strains overexpressing M.
tb chimeric antigen Ag856A2 (coding a recombinant ag85a with 2 copies of esat-6 inserted at Acc I site of ag85a) at differential levels under the control of the subtly modified furA promoters.
These two rBCG strains were used to vaccinate C57BL/6 mice and exploit dose of incorporated antigen in rBCG to optimize immune response and protective efficiency against M.
tb challenge in mouse model.
The results showed that rBCG strains overexpressing Ag856A2 at differential levels induced different antigen-specific IFN-γ production and comparable number of M.
tb-specific CD4 T cells expressing IL-2.
M.
tb challenge experiment showed that rBCG strains afforded enhanced but comparable immune protection characterized by reduced bacillary load, lung pathology, and inflammation.
These results suggested that the dose of antigens incorporated in rBCG can impact T cell immune responses but imposed no significantly differential protective efficacies.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ma, Hui& Wu, Kang& Liu, Fang& Yang, Hua& Kang, Han& Chen, Ning-Ning…[et al.]. 2014. Dose of Incorporated Immunodominant Antigen in Recombinant BCG Impacts Modestly on Th1 Immune Response and Protective Efficiency against Mycobacterium tuberculosis in Mice. Journal of Immunology Research،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1040788
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ma, Hui…[et al.]. Dose of Incorporated Immunodominant Antigen in Recombinant BCG Impacts Modestly on Th1 Immune Response and Protective Efficiency against Mycobacterium tuberculosis in Mice. Journal of Immunology Research No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1040788
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ma, Hui& Wu, Kang& Liu, Fang& Yang, Hua& Kang, Han& Chen, Ning-Ning…[et al.]. Dose of Incorporated Immunodominant Antigen in Recombinant BCG Impacts Modestly on Th1 Immune Response and Protective Efficiency against Mycobacterium tuberculosis in Mice. Journal of Immunology Research. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1040788
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1040788
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر