Dose of Incorporated Immunodominant Antigen in Recombinant BCG Impacts Modestly on Th1 Immune Response and Protective Efficiency against Mycobacterium tuberculosis in Mice
Joint Authors
Wu, Kang
Fan, Xiao-Yong
Ma, Hui
Liu, Fang
Yang, Hua
Kang, Han
Chen, Ning-Ning
Yuan, Qin
Zhou, Wen-Jiang
Source
Journal of Immunology Research
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-23
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
One approach for improving BCG efficacy is to utilize BCG as vehicle to develop recombinant BCG (rBCG) strains overexpressing Mycobacterium tuberculosis (M.
tb) antigens.
Also expression level of a candidate antigen should impact the final T cell responses conferred by rBCG.
In this study, based on our previously constructed differential expression system, we developed two rBCG strains overexpressing M.
tb chimeric antigen Ag856A2 (coding a recombinant ag85a with 2 copies of esat-6 inserted at Acc I site of ag85a) at differential levels under the control of the subtly modified furA promoters.
These two rBCG strains were used to vaccinate C57BL/6 mice and exploit dose of incorporated antigen in rBCG to optimize immune response and protective efficiency against M.
tb challenge in mouse model.
The results showed that rBCG strains overexpressing Ag856A2 at differential levels induced different antigen-specific IFN-γ production and comparable number of M.
tb-specific CD4 T cells expressing IL-2.
M.
tb challenge experiment showed that rBCG strains afforded enhanced but comparable immune protection characterized by reduced bacillary load, lung pathology, and inflammation.
These results suggested that the dose of antigens incorporated in rBCG can impact T cell immune responses but imposed no significantly differential protective efficacies.
American Psychological Association (APA)
Ma, Hui& Wu, Kang& Liu, Fang& Yang, Hua& Kang, Han& Chen, Ning-Ning…[et al.]. 2014. Dose of Incorporated Immunodominant Antigen in Recombinant BCG Impacts Modestly on Th1 Immune Response and Protective Efficiency against Mycobacterium tuberculosis in Mice. Journal of Immunology Research،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1040788
Modern Language Association (MLA)
Ma, Hui…[et al.]. Dose of Incorporated Immunodominant Antigen in Recombinant BCG Impacts Modestly on Th1 Immune Response and Protective Efficiency against Mycobacterium tuberculosis in Mice. Journal of Immunology Research No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1040788
American Medical Association (AMA)
Ma, Hui& Wu, Kang& Liu, Fang& Yang, Hua& Kang, Han& Chen, Ning-Ning…[et al.]. Dose of Incorporated Immunodominant Antigen in Recombinant BCG Impacts Modestly on Th1 Immune Response and Protective Efficiency against Mycobacterium tuberculosis in Mice. Journal of Immunology Research. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1040788
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1040788