Pathological and ultrastructural studies anisakis simplex rudolphi-1809 infecting carangoides bajad with special reference to intestinal maturation in puppies

الملخص EN

A total of 120 albino mice were divided into: Gl (80 infected with S.

manso-ni cercariae by tail immersion) and G2 (40 uninfected controls).

Seven weeks after infection, Gl was subdivided into four subgroups of 20 mice each.

Gla: Infected untreated control.

Gib: treated with Praziquantel (PZQ) in a dose of 500 mg/kg for two consecutive days.

Glc: treated with Oltipraz (Oz) in a dose of 500 mg/kg for three consecutive days.

Gld: treated with combination of PZQ and Oz (1/3 therapeutic dose of both for two consecutive days.

G2 was divided into 4 subgroups of ten mice each.

G2a: non infected non treated.

G2b: PZQ treated (PZQ control positive).

G2c: Oz treated (Oz control positive).

G2d: Drugs treated (PZQ and Oz control positive).

The effect of drugs was done 2 weeks post-treatment, parasitological to detect number of worms and the distribution in liver vasculature after perfusion of hepatic circulation of mice, and number of eggs per gram of liver tissue.

Al¬so, histopathological examination of liver samples with H& E and/or Masson's trachoma stains showed effect on granuloma and tissue.

PZQ gave reduction of .

total worm burden to 0.22 +0.01 (99%), while Oz gave 22.4+0.57 (3.6%).

PZQ produced marked hepatic shift while Oz gave 6.14%efficacy.

A decrease in egg number with PZQ was 63.69%.

Oz gave 13.5%.

Complete disappearance of immature ova with increase no.

of dead ones was 97.89% of mature ova stage to 2.11% after PZQ.

Oz gave 45% of all detected ova with slight increase of dead ones to 14.18% compared to 9.8% in infected control.

Improvement of hepatic pathology in form of 51.2% reduction in granuloma size and 54.7% decrease in number with improvement of hepatocytes was after treatment with PZQ in contrary to Oz as reduction of size and number of granulorpa was 13.6% and 21.6% respectively.

Combination of reduced dose of both drugs produced moderate effect on all criteria less than that detected with full dose of PZQ, but much better that obtained after Oz.