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Simultaneous Detection and Differentiation of Highly Virulent and Classical Chinese-Type Isolation of PRRSV by Real-Time RT-PCR
المؤلفون المشاركون
Xiao, Shuqi
Chen, Yaosheng
Wang, Liangliang
Gao, Jintao
Mo, Delin
He, Zuyong
Liu, Xiaohong
المصدر
Journal of Immunology Research
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-06-12
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Porcine reproductive and respiratory syndrome (PRRS) is a leading disease in pig industry worldwide and can result in serious economic losses each year.
The PRRS epidemic situation in China has been very complicated since the unprecedented large-scale highly pathogenic PRRS (HP-PRRS) outbreaks in 2006.
And now the HP-PRRS virus (HP-PRRSV) and classical North American type PRRSV strains have coexisted in China.
Rapid differential detection of the two strains of PRRSV is very important for effective PRRS control.
The real-time RT-PCR for simultaneous detection and differentiation of HP-PRRSV and PRRSV by using both SYBR Green and TaqMan probes was developed and validated.
Both assays can be used for rapid detection and strain-specific identification of HP-PRRSV and PRRSV.
However, the TaqMan probe method had the highest detection rate whereas the conventional RT-PCR was the lowest.
The real-time RT-PCR developed based on SYBR Green and TaqMan probe could be used for simultaneous detection and differentiation of HP-PRRSV and PRRSV in China, which will benefit much the PRRS control and research.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Xiao, Shuqi& Chen, Yaosheng& Wang, Liangliang& Gao, Jintao& Mo, Delin& He, Zuyong…[et al.]. 2014. Simultaneous Detection and Differentiation of Highly Virulent and Classical Chinese-Type Isolation of PRRSV by Real-Time RT-PCR. Journal of Immunology Research،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1040988
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Xiao, Shuqi…[et al.]. Simultaneous Detection and Differentiation of Highly Virulent and Classical Chinese-Type Isolation of PRRSV by Real-Time RT-PCR. Journal of Immunology Research No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-1040988
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Xiao, Shuqi& Chen, Yaosheng& Wang, Liangliang& Gao, Jintao& Mo, Delin& He, Zuyong…[et al.]. Simultaneous Detection and Differentiation of Highly Virulent and Classical Chinese-Type Isolation of PRRSV by Real-Time RT-PCR. Journal of Immunology Research. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1040988
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1040988
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