Simultaneous Detection and Differentiation of Highly Virulent and Classical Chinese-Type Isolation of PRRSV by Real-Time RT-PCR

Abstract EN

Porcine reproductive and respiratory syndrome (PRRS) is a leading disease in pig industry worldwide and can result in serious economic losses each year.

The PRRS epidemic situation in China has been very complicated since the unprecedented large-scale highly pathogenic PRRS (HP-PRRS) outbreaks in 2006.

And now the HP-PRRS virus (HP-PRRSV) and classical North American type PRRSV strains have coexisted in China.

Rapid differential detection of the two strains of PRRSV is very important for effective PRRS control.

The real-time RT-PCR for simultaneous detection and differentiation of HP-PRRSV and PRRSV by using both SYBR Green and TaqMan probes was developed and validated.

Both assays can be used for rapid detection and strain-specific identification of HP-PRRSV and PRRSV.

However, the TaqMan probe method had the highest detection rate whereas the conventional RT-PCR was the lowest.

The real-time RT-PCR developed based on SYBR Green and TaqMan probe could be used for simultaneous detection and differentiation of HP-PRRSV and PRRSV in China, which will benefit much the PRRS control and research.