Morphological Features and In Vitro Cytopathic Effect of Acanthamoeba griffini Trophozoites Isolated from a Clinical Case
المؤلفون المشاركون
Omaña-Molina, Maritza
Salazar-Villatoro, Lizbeth
Reyes-Batlle, Maria
Martín-Navarro, Carmen M.
Lorenzo-Morales, Jacob
González-Robles, Arturo
المصدر
Journal of Parasitology Research
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-09-08
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Light and transmission electron microscopy observations are reported on the structure and in vitro cytopathic effect of Acanthamoeba griffini trophozoites isolated from a clinical case.
Live trophozoites were moderately active with a remarkable pleomorphism which changed from ovoid to quite elongated shapes.
When moving, amoebae formed cytoplasmic projections such as wide lamellae and acanthopodia of diverse size and thickness which contain a significant amount of actin.
Ultrastructurally, the cytoplasm showed the main organelles found in other free-living amoebae.
Coincubation of trophozoites with MDCK cell monolayers resulted in a local damage to target cells after 24 h of interaction, suggesting that the cytopathic effect is contact-dependent.
By transmission electron microscopy, amoebae appeared to engulf small portions of the MDCK cells; however, the cells that were not in contact with trophozoites had an unaltered morphology.
When epithelial monolayers were incubated with conditioned medium for 24 h, small areas of cell injury were also observed.
The phylogenetical analysis as well as the sequencing of the acquired amplified product for the DF3 region of the amoebae isolate confirmed that it belongs to genotype T3, which includes other pathogenic amoebae; besides the activity of two drugs currently used against Acanthamoeba was tested on A.
griffini.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
González-Robles, Arturo& Salazar-Villatoro, Lizbeth& Omaña-Molina, Maritza& Reyes-Batlle, Maria& Martín-Navarro, Carmen M.& Lorenzo-Morales, Jacob. 2014. Morphological Features and In Vitro Cytopathic Effect of Acanthamoeba griffini Trophozoites Isolated from a Clinical Case. Journal of Parasitology Research،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1042786
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
González-Robles, Arturo…[et al.]. Morphological Features and In Vitro Cytopathic Effect of Acanthamoeba griffini Trophozoites Isolated from a Clinical Case. Journal of Parasitology Research No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1042786
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
González-Robles, Arturo& Salazar-Villatoro, Lizbeth& Omaña-Molina, Maritza& Reyes-Batlle, Maria& Martín-Navarro, Carmen M.& Lorenzo-Morales, Jacob. Morphological Features and In Vitro Cytopathic Effect of Acanthamoeba griffini Trophozoites Isolated from a Clinical Case. Journal of Parasitology Research. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1042786
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1042786
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر