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Morphological Features and In Vitro Cytopathic Effect of Acanthamoeba griffini Trophozoites Isolated from a Clinical Case
Joint Authors
Omaña-Molina, Maritza
Salazar-Villatoro, Lizbeth
Reyes-Batlle, Maria
Martín-Navarro, Carmen M.
Lorenzo-Morales, Jacob
González-Robles, Arturo
Source
Journal of Parasitology Research
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-09-08
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Light and transmission electron microscopy observations are reported on the structure and in vitro cytopathic effect of Acanthamoeba griffini trophozoites isolated from a clinical case.
Live trophozoites were moderately active with a remarkable pleomorphism which changed from ovoid to quite elongated shapes.
When moving, amoebae formed cytoplasmic projections such as wide lamellae and acanthopodia of diverse size and thickness which contain a significant amount of actin.
Ultrastructurally, the cytoplasm showed the main organelles found in other free-living amoebae.
Coincubation of trophozoites with MDCK cell monolayers resulted in a local damage to target cells after 24 h of interaction, suggesting that the cytopathic effect is contact-dependent.
By transmission electron microscopy, amoebae appeared to engulf small portions of the MDCK cells; however, the cells that were not in contact with trophozoites had an unaltered morphology.
When epithelial monolayers were incubated with conditioned medium for 24 h, small areas of cell injury were also observed.
The phylogenetical analysis as well as the sequencing of the acquired amplified product for the DF3 region of the amoebae isolate confirmed that it belongs to genotype T3, which includes other pathogenic amoebae; besides the activity of two drugs currently used against Acanthamoeba was tested on A.
griffini.
American Psychological Association (APA)
González-Robles, Arturo& Salazar-Villatoro, Lizbeth& Omaña-Molina, Maritza& Reyes-Batlle, Maria& Martín-Navarro, Carmen M.& Lorenzo-Morales, Jacob. 2014. Morphological Features and In Vitro Cytopathic Effect of Acanthamoeba griffini Trophozoites Isolated from a Clinical Case. Journal of Parasitology Research،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1042786
Modern Language Association (MLA)
González-Robles, Arturo…[et al.]. Morphological Features and In Vitro Cytopathic Effect of Acanthamoeba griffini Trophozoites Isolated from a Clinical Case. Journal of Parasitology Research No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1042786
American Medical Association (AMA)
González-Robles, Arturo& Salazar-Villatoro, Lizbeth& Omaña-Molina, Maritza& Reyes-Batlle, Maria& Martín-Navarro, Carmen M.& Lorenzo-Morales, Jacob. Morphological Features and In Vitro Cytopathic Effect of Acanthamoeba griffini Trophozoites Isolated from a Clinical Case. Journal of Parasitology Research. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1042786
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1042786