![](/images/graphics-bg.png)
MHC-DRB1DQB1 Gene Polymorphism and Its Association with ResistanceSusceptibility to Cystic Echinococcosis in Chinese Merino Sheep
المؤلفون المشاركون
Shen, Hong
Han, Guohua
Jia, Bin
Jiang, Song
Du, Yingchun
المصدر
Journal of Parasitology Research
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-03-24
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
The aim of this study was to analyze the relationship between polymorphism of the MHC-DRB1/DQB1 gene and its resistance to Cystic Echinococcosis (C.E), as well as to screen out the molecular genetic marker of antiechinococcosis in Chinese Merino sheep.
The MHCII-DRB1/DQB1 exon 2 was amplified by polymerase chain reaction (PCR) from DNA samples of healthy and hydatidosis sheep.
PCR products were characterized by restriction fragment length polymorphism (RFLP) technique.
Five restriction enzymes (Mval, HaeIII, SacI, SacII, and Hin1I) were employed to cut DRB1, while seven restriction enzymes (MroxI, ScaI, SacII, NciI, TaqI, Mval, and HaeIII) were employed to cut DQB1.Results showed that frequencies of patterns Mvalbb ( P < 0.01 ), SacIab in DRB1 exon 2 ( P < 0.05 ), and TaqIaa, HaeIIInn ( P < 0.01 ) in DQB1 exon 2 were significantly higher in the healthy group compared with the C.E individuals, which implied that there was a strong association between these genotypes and hydatidosis resistance or susceptibility.
Chi-square test showed that individuals with the genic haplotype DRB1-SacIab/DRB1-Mvalbb/DQB1-TaqIaa/DQB1-HaeIIInn ( P < 0.01 ) were relatively resistant to C.E, while individuals with the genic haplotypes DRB1-Mvalbc/DQB1-Mvalyy/DQB1-TaqIab/DQB1-HaeIIImn ( P < 0.01 ) and DRB1-Mvalbb/DQB1-Mvalcc/DQB1-TaqIab/DQB1-HaeIIImn ( P < 0.01 ) were more susceptible to C.E.
In addition, to confirm these results, a fielding experiment was performed with Chinese Merino sheep which were artificially infected with E.g.
The result was in accordance with the results of the first study.
In conclusion, MHC-DRB1/DQB1 exon 2 plays an important role as resistant to C.E in Chinese Merino sheep.
In addition, the molecular genetic marker of antiechinococcosis (DRB1-SacIab/DRB1-Mvalbb/DQB1-TaqIaa/DQB1-HaeIIInn) was screened out in Chinese Merino sheep.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Shen, Hong& Han, Guohua& Jia, Bin& Jiang, Song& Du, Yingchun. 2014. MHC-DRB1DQB1 Gene Polymorphism and Its Association with ResistanceSusceptibility to Cystic Echinococcosis in Chinese Merino Sheep. Journal of Parasitology Research،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1042787
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Han, Guohua…[et al.]. MHC-DRB1DQB1 Gene Polymorphism and Its Association with ResistanceSusceptibility to Cystic Echinococcosis in Chinese Merino Sheep. Journal of Parasitology Research No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-1042787
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Shen, Hong& Han, Guohua& Jia, Bin& Jiang, Song& Du, Yingchun. MHC-DRB1DQB1 Gene Polymorphism and Its Association with ResistanceSusceptibility to Cystic Echinococcosis in Chinese Merino Sheep. Journal of Parasitology Research. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1042787
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1042787
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)