MHC-DRB1DQB1 Gene Polymorphism and Its Association with ResistanceSusceptibility to Cystic Echinococcosis in Chinese Merino Sheep
Joint Authors
Shen, Hong
Han, Guohua
Jia, Bin
Jiang, Song
Du, Yingchun
Source
Journal of Parasitology Research
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-24
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The aim of this study was to analyze the relationship between polymorphism of the MHC-DRB1/DQB1 gene and its resistance to Cystic Echinococcosis (C.E), as well as to screen out the molecular genetic marker of antiechinococcosis in Chinese Merino sheep.
The MHCII-DRB1/DQB1 exon 2 was amplified by polymerase chain reaction (PCR) from DNA samples of healthy and hydatidosis sheep.
PCR products were characterized by restriction fragment length polymorphism (RFLP) technique.
Five restriction enzymes (Mval, HaeIII, SacI, SacII, and Hin1I) were employed to cut DRB1, while seven restriction enzymes (MroxI, ScaI, SacII, NciI, TaqI, Mval, and HaeIII) were employed to cut DQB1.Results showed that frequencies of patterns Mvalbb ( P < 0.01 ), SacIab in DRB1 exon 2 ( P < 0.05 ), and TaqIaa, HaeIIInn ( P < 0.01 ) in DQB1 exon 2 were significantly higher in the healthy group compared with the C.E individuals, which implied that there was a strong association between these genotypes and hydatidosis resistance or susceptibility.
Chi-square test showed that individuals with the genic haplotype DRB1-SacIab/DRB1-Mvalbb/DQB1-TaqIaa/DQB1-HaeIIInn ( P < 0.01 ) were relatively resistant to C.E, while individuals with the genic haplotypes DRB1-Mvalbc/DQB1-Mvalyy/DQB1-TaqIab/DQB1-HaeIIImn ( P < 0.01 ) and DRB1-Mvalbb/DQB1-Mvalcc/DQB1-TaqIab/DQB1-HaeIIImn ( P < 0.01 ) were more susceptible to C.E.
In addition, to confirm these results, a fielding experiment was performed with Chinese Merino sheep which were artificially infected with E.g.
The result was in accordance with the results of the first study.
In conclusion, MHC-DRB1/DQB1 exon 2 plays an important role as resistant to C.E in Chinese Merino sheep.
In addition, the molecular genetic marker of antiechinococcosis (DRB1-SacIab/DRB1-Mvalbb/DQB1-TaqIaa/DQB1-HaeIIInn) was screened out in Chinese Merino sheep.
American Psychological Association (APA)
Shen, Hong& Han, Guohua& Jia, Bin& Jiang, Song& Du, Yingchun. 2014. MHC-DRB1DQB1 Gene Polymorphism and Its Association with ResistanceSusceptibility to Cystic Echinococcosis in Chinese Merino Sheep. Journal of Parasitology Research،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1042787
Modern Language Association (MLA)
Han, Guohua…[et al.]. MHC-DRB1DQB1 Gene Polymorphism and Its Association with ResistanceSusceptibility to Cystic Echinococcosis in Chinese Merino Sheep. Journal of Parasitology Research No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-1042787
American Medical Association (AMA)
Shen, Hong& Han, Guohua& Jia, Bin& Jiang, Song& Du, Yingchun. MHC-DRB1DQB1 Gene Polymorphism and Its Association with ResistanceSusceptibility to Cystic Echinococcosis in Chinese Merino Sheep. Journal of Parasitology Research. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1042787
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1042787