A Comparison of Nested PCR Assay with Conventional Techniques for Diagnosis of Intestinal Cryptosporidiosis in AIDS Cases from Northern India
المؤلفون المشاركون
Jha, Arun Kumar
Singh, Ompal
Uppal, Beena
Chadha, Sanjim
المصدر
Journal of Parasitology Research
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-01-12
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Cryptosporidiosis is a very important opportunistic infection and is responsible for significant morbidity and mortality in HIV/AIDS patients.
Although current laboratory methods are generally considered adequate to detect high concentrations of oocysts, they fail to detect cases of cryptosporidiosis in many immunocompromised patients.
The present study was done to determine the diagnostic efficacy of modified Ziehl-Neelsen (ZN), antigen detection ELISA, and a nested PCR assay for detection of Cryptosporidium in 58 adult AIDS cases with diarrhea from the ART clinic of Lok Nayak Hospital, New Delhi.
Cryptosporidium was detected in 17 (29.4%), 39 (67.3%), and 45 (77.5%) cases by modified ZN staining, antigen ELISA, and nested PCR assay, respectively.
Taking nested PCR as the gold standard, specificity of both modified ZN staining and Cryptosporidium antigen detection ELISA was 100% while the sensitivity of the tests was 37.8% and 86.6%, respectively.
PCR was more sensitive than the other two diagnostic modalities but required a more hands-on time per sample and was more expensive than microscopy.
PCR, however, was very adaptable to batch analysis, reducing the costs considerably.
This assay can therefore have considerable advantages in the treatment of immunosuppressed individuals like AIDS patients, allowing their early diagnosis and decreasing the morbidity and the mortality.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Uppal, Beena& Singh, Ompal& Chadha, Sanjim& Jha, Arun Kumar. 2014. A Comparison of Nested PCR Assay with Conventional Techniques for Diagnosis of Intestinal Cryptosporidiosis in AIDS Cases from Northern India. Journal of Parasitology Research،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1042801
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Uppal, Beena…[et al.]. A Comparison of Nested PCR Assay with Conventional Techniques for Diagnosis of Intestinal Cryptosporidiosis in AIDS Cases from Northern India. Journal of Parasitology Research No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-1042801
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Uppal, Beena& Singh, Ompal& Chadha, Sanjim& Jha, Arun Kumar. A Comparison of Nested PCR Assay with Conventional Techniques for Diagnosis of Intestinal Cryptosporidiosis in AIDS Cases from Northern India. Journal of Parasitology Research. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1042801
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1042801
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر