A Comparison of Nested PCR Assay with Conventional Techniques for Diagnosis of Intestinal Cryptosporidiosis in AIDS Cases from Northern India
Joint Authors
Jha, Arun Kumar
Singh, Ompal
Uppal, Beena
Chadha, Sanjim
Source
Journal of Parasitology Research
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-12
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Cryptosporidiosis is a very important opportunistic infection and is responsible for significant morbidity and mortality in HIV/AIDS patients.
Although current laboratory methods are generally considered adequate to detect high concentrations of oocysts, they fail to detect cases of cryptosporidiosis in many immunocompromised patients.
The present study was done to determine the diagnostic efficacy of modified Ziehl-Neelsen (ZN), antigen detection ELISA, and a nested PCR assay for detection of Cryptosporidium in 58 adult AIDS cases with diarrhea from the ART clinic of Lok Nayak Hospital, New Delhi.
Cryptosporidium was detected in 17 (29.4%), 39 (67.3%), and 45 (77.5%) cases by modified ZN staining, antigen ELISA, and nested PCR assay, respectively.
Taking nested PCR as the gold standard, specificity of both modified ZN staining and Cryptosporidium antigen detection ELISA was 100% while the sensitivity of the tests was 37.8% and 86.6%, respectively.
PCR was more sensitive than the other two diagnostic modalities but required a more hands-on time per sample and was more expensive than microscopy.
PCR, however, was very adaptable to batch analysis, reducing the costs considerably.
This assay can therefore have considerable advantages in the treatment of immunosuppressed individuals like AIDS patients, allowing their early diagnosis and decreasing the morbidity and the mortality.
American Psychological Association (APA)
Uppal, Beena& Singh, Ompal& Chadha, Sanjim& Jha, Arun Kumar. 2014. A Comparison of Nested PCR Assay with Conventional Techniques for Diagnosis of Intestinal Cryptosporidiosis in AIDS Cases from Northern India. Journal of Parasitology Research،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1042801
Modern Language Association (MLA)
Uppal, Beena…[et al.]. A Comparison of Nested PCR Assay with Conventional Techniques for Diagnosis of Intestinal Cryptosporidiosis in AIDS Cases from Northern India. Journal of Parasitology Research No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-1042801
American Medical Association (AMA)
Uppal, Beena& Singh, Ompal& Chadha, Sanjim& Jha, Arun Kumar. A Comparison of Nested PCR Assay with Conventional Techniques for Diagnosis of Intestinal Cryptosporidiosis in AIDS Cases from Northern India. Journal of Parasitology Research. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1042801
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1042801